The expression of RANTES and its monocyte chemotactic activity in the stromal cells of autologous eutopic endometrium of the patients with endometriosis

Objectives: To evaluate if the expression and chemotactic activity of RANTES are different in IL-1β treated autologous eutopic endometrial stromal cells from endometriosis patients compared to non-endometriosis eutopic endometrium.Methods: Eutopic endometrial stromal cells from patients with or without endometriosis were collected and cultured.IL-1β was added to induce the secretion of RANTES.Concentrations of RANTES in the culture medium at 12 h,24 h,36 h,48 h,60 h,72 h,84 h and 96 h were measured with a sandwich ELISA.U937 monocyte chemotactic activity was assayed in Boyden Chambers.Results: The RANTES secreted by the stromal cells of autologous eutopic endometrium from endometriosis patients was increased significantly than that from non-endometriosis women after 60 h of culture.The RANTES accounts for the majority(55%) of the monocyte chemotactic activity in autologous eutopic endometrial stromal cells conditioned media. Conclusions: The expression of RANTES and its monocyte chemotactic activity of autologous eutopic endometrium in the endometriosis patients were different significantly from those in non-endometriosis women.