Gastrointestinal parasites of zoonotic importance detected in bats in the conservation area of Semuliki National Park, western Uganda

Abstract Bat guano may contain zoonotic parasites that contaminate the environment and/or serve as a potential source of infection to humans and animals. Repeated bat-human exposure could be a risk factor for zoonosis. To date, knowledge on the status of bat gastrointestinal parasites (GIPs) in Uganda is limited. We conducted a cross-sectional study to investigate the prevalence of bat GIP species in communities contiguous to Semuliki National Park (SNP) Bundibugyo district in western Uganda. We purposively collected faecal samples of micro- (n=242) and mega-bats (n=242) from bat roosts in communities contiguous to SNP during the rainy months of October to December 2023 and the dry months of January to March 2024. Standard faecal floatation and sedimentation techniques were used for laboratory examination. Microscopic examination revealed that 224 (46%) samples tested positive for more than one parasite species. Thirteen GIPs, including protozoa (n=3), trematode (n=1), cestode (n=1), and nematodes (n=8) were detected. The most prevalent parasites were Entamoeba coli (57%), Hookworm (33%), Strongyloides sp. (33%), and E. histolytica (32%), and the least prevalent were the two Unidentified nematodes (1%). 77% (n=10) of the detected GIPs are responsible for zoonosis and are of significant public health importance. Statistically, there was a significant difference (P < 0.05) in the overall parasite prevalence across the four studied bat groups. Also, parasite prevalence was significantly higher in microbats compared to megabats (P < 0.05) and in Burondo sub-county compared to Ntandi town council (P< 0.05). The detected zoonotic parasites pose a potential source of zoonosis in communities contiguous to the conservation area of Semuliki National Park, Uganda. This calls for awareness creation on the risks of bat mediated zoonotic parasitosis and the use of good sanitary practices to prevent chances of zoonotic parasite spillover from bats to humans. Competing Interest Statement The authors have declared no competing interest. Funding Statement his study did not receive any funding Author Declarations I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained. Yes I confirm that all necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived, and that any patient/participant/sample identifiers included were not known to anyone (e.g., hospital staff, patients or participants themselves) outside the research group so cannot be used to identify individuals. Yes I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance). Yes I have followed all appropriate research reporting guidelines, such as any relevant EQUATOR Network research reporting checklist(s) and other pertinent material, if applicable. Yes Data Availability All data produced in the present study are available upon reasonable request to the authors