Alleviation of Pepper Continuous Cropping Obstacles by Chaetomium globosum CEF-082 Through Rhizosphere Microbiome Manipulation

Alleviation of Pepper Continuous Cropping Obstacles by Chaetomium globosum CEF-082 Through Rhizosphere Microbiome Manipulation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Alleviation of Pepper Continuous Cropping Obstacles by Chaetomium globosum CEF-082 Through Rhizosphere Microbiome Manipulation Peizhen DING, Jinglong ZHOU, Feng WEI, Yalin ZHANG, Lihong ZHAO, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8023939/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 03 Mar, 2026 Read the published version in Plant and Soil → Version 1 posted 6 You are reading this latest preprint version Abstract Aims The inhibitory effect and mechanism of the biological control fungus Chaetomium globosum CEF-082 on pepper Continuous Cropping Obstacles (CCOs) were investigated. Specifically, it evaluated the effect of Chaetomium globosum CEF-082 on the composition structure of the soil microbial community in the rhizosphere of chili peppers. Methods Rhizosphere soil samples were collected from farmlands with continuous cropping for 3 years (Y3) and 20 years (Y20). Chaetomium globosum CEF-082 spore solution was treated in a greenhouse, and antagonism experiments were conducted on solid medium of glucose potato. Results The results indicated that prolonged planting led to severe soil nutrient loss, a 94.2% decrease in total nitrogen content, and a significant increase in the abundance of the pathogenic bacterium Fusarium. In vitro experiments confirmed that CEF-082 has a strong antagonistic activity against the main pathogenic bacteria of Fusarium. Greenhouse pot experiments have shown that the application of CEF-082 can significantly reduce the mortality rate of seedlings and improve the growth parameters of plants. High-throughput sequencing results showed that CEF-082 reshaped the rhizospheric microbial community, reduced the relative abundance of Fusarium, and might promote the colonization of beneficial Trichoderma species. Conclusions We believe that C. globosum CEF-082 alleviates CCOs through multiple mechanisms such as direct antagonism, regulation of rhizosphere microbiota, and improvement of soil nutrient availability. This study provides a sustainable strategy based on the microbiota for the management of soil-borne diseases in solanaceous crops. Continuous Cropping Obstacles soil microbial communities soil physicochemical properties microbiome assembly Full Text Cite Share Download PDF Status: Published Journal Publication published 03 Mar, 2026 Read the published version in Plant and Soil → Version 1 posted Editorial decision: Minor revisions 29 Jan, 2026 Reviewers agreed at journal 14 Dec, 2025 Reviewers invited by journal 07 Dec, 2025 Editor invited by journal 27 Nov, 2025 Editor assigned by journal 26 Nov, 2025 First submitted to journal 20 Nov, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8023939","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":556459406,"identity":"9fe048d5-610d-4a9b-a22a-55a48f732432","order_by":0,"name":"Peizhen DING","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Peizhen","middleName":"","lastName":"DING","suffix":""},{"id":556459407,"identity":"86741465-5987-4ff2-919e-358dcac7ac1e","order_by":1,"name":"Jinglong 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Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Continuous Cropping Obstacles, soil microbial communities, soil physicochemical properties, microbiome assembly","lastPublishedDoi":"10.21203/rs.3.rs-8023939/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8023939/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eAims\u003c/h2\u003e\u003cp\u003eThe inhibitory effect and mechanism of the biological control fungus Chaetomium globosum CEF-082 on pepper Continuous Cropping Obstacles (CCOs) were investigated. Specifically, it evaluated the effect of Chaetomium globosum CEF-082 on the composition structure of the soil microbial community in the rhizosphere of chili peppers.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e\u003cp\u003eRhizosphere soil samples were collected from farmlands with continuous cropping for 3 years (Y3) and 20 years (Y20). Chaetomium globosum CEF-082 spore solution was treated in a greenhouse, and antagonism experiments were conducted on solid medium of glucose potato.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e\u003cp\u003eThe results indicated that prolonged planting led to severe soil nutrient loss, a 94.2% decrease in total nitrogen content, and a significant increase in the abundance of the pathogenic bacterium Fusarium. In vitro experiments confirmed that CEF-082 has a strong antagonistic activity against the main pathogenic bacteria of Fusarium. Greenhouse pot experiments have shown that the application of CEF-082 can significantly reduce the mortality rate of seedlings and improve the growth parameters of plants. High-throughput sequencing results showed that CEF-082 reshaped the rhizospheric microbial community, reduced the relative abundance of Fusarium, and might promote the colonization of beneficial Trichoderma species.\u003c/p\u003e\u003ch2\u003eConclusions\u003c/h2\u003e\u003cp\u003eWe believe that C. globosum CEF-082 alleviates CCOs through multiple mechanisms such as direct antagonism, regulation of rhizosphere microbiota, and improvement of soil nutrient availability. This study provides a sustainable strategy based on the microbiota for the management of soil-borne diseases in solanaceous crops.\u003c/p\u003e","manuscriptTitle":"Alleviation of Pepper Continuous Cropping Obstacles by Chaetomium globosum CEF-082 Through Rhizosphere Microbiome Manipulation","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-12-11 09:16:41","doi":"10.21203/rs.3.rs-8023939/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Minor revisions","date":"2026-01-29T10:29:50+00:00","index":"","fulltext":""},{"type":"reviewerAgreed","content":"","date":"2025-12-14T18:12:30+00:00","index":0,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-12-07T11:51:50+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"Plant and Soil","date":"2025-11-27T06:37:19+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-11-27T03:07:30+00:00","index":"","fulltext":""},{"type":"submitted","content":"Plant and Soil","date":"2025-11-21T03:32:12+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"plant-and-soil","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"plso","sideBox":"Learn more about [Plant and Soil](https://www.springer.com/journal/11104)","snPcode":"11104","submissionUrl":"https://submission.nature.com/new-submission/11104/3","title":"Plant and Soil","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"f0a31b5c-eee0-407a-a5b7-6d5061e80fab","owner":[],"postedDate":"December 11th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2026-03-09T16:08:40+00:00","versionOfRecord":{"articleIdentity":"rs-8023939","link":"https://doi.org/10.1007/s11104-026-08414-w","journal":{"identity":"plant-and-soil","isVorOnly":false,"title":"Plant and Soil"},"publishedOn":"2026-03-03 15:57:45","publishedOnDateReadable":"March 3rd, 2026"},"versionCreatedAt":"2025-12-11 09:16:41","video":"","vorDoi":"10.1007/s11104-026-08414-w","vorDoiUrl":"https://doi.org/10.1007/s11104-026-08414-w","workflowStages":[]},"version":"v1","identity":"rs-8023939","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8023939","identity":"rs-8023939","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}