Evaluation of Antioxidant and Spectroscopic analysis of Psoralea corylifolia and Nigella sativa Plant Extract

Evaluation of Antioxidant and Spectroscopic analysis of Psoralea corylifolia and Nigella sativa Plant Extract | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Evaluation of Antioxidant and Spectroscopic analysis of Psoralea corylifolia and Nigella sativa Plant Extract Rohit Agrahari, Guno Sindhu Chakraborthy This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7750071/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Analytical methods of UV spectroscopy, FTIR, TLC and antioxidant activity are used to test the drug and active constituent in the laboratory to determine how much active constituent is present in the test drug through these methods. Aim In UV estimation, the test at 254 nm has a very sharp peak, which represents that the test drug is pure, which shows that this nm is present in the psoralene active constituent that is present in the psoralene corylifolia and In FTIR analysis of the sample, how many functional groups are present in the test sample. Methods In TLC analysis, a different mobile phase was prepared, and all the samples were run, but in one phase, the test samples ran excellently, and separation was good (hexane: ethyl acetate) in a ratio of 8.5:1.5 v/v. Compared to the test drug with an active constituent, the RF valve is the same. Result In antioxidant activity, antioxidant properties with DPPH and compare them to the standard antioxidant drug, Gallic acid. In this activity we prepare the different concentrations of the solution and check which concentration shows the high antioxidant activity and compare it with the standard drug concentration activity. Conclusion The test drug antioxidant property is high in the 100 µL concentration. The sample is run in UV-visible spectroscopy at 517 nm, and the standard is run at 270nm for the absorbance of the drug. Major Finding: The study revealed that both Psoralea corylifolia and Nigella sativa extracts exhibit strong antioxidant activity, with Nigella sativa showing slightly higher free radical scavenging potential. Spectroscopic analysis confirmed the presence of phenolic and flavonoid compounds contributing to their antioxidant properties. UV Spectroscopy FTIR DPPH activity Herbal drug Analysis Analysis Vitiligo Inflammatory bowel disease Thymoquinone Bakuchiol Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Extract","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"UV Spectroscopy, FTIR, DPPH activity, Herbal drug Analysis, Analysis, Vitiligo, Inflammatory bowel disease, Thymoquinone, Bakuchiol","lastPublishedDoi":"10.21203/rs.3.rs-7750071/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7750071/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e\u003cp\u003eAnalytical methods of UV spectroscopy, FTIR, TLC and antioxidant activity are used to test the drug and active constituent in the laboratory to determine how much active constituent is present in the test drug through these methods.\u003c/p\u003e\u003ch2\u003eAim\u003c/h2\u003e\u003cp\u003eIn UV estimation, the test at 254 nm has a very sharp peak, which represents that the test drug is pure, which shows that this nm is present in the psoralene active constituent that is present in the \u003cem\u003epsoralene corylifolia\u003c/em\u003e and In FTIR analysis of the sample, how many functional groups are present in the test sample.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e\u003cp\u003eIn TLC analysis, a different mobile phase was prepared, and all the samples were run, but in one phase, the test samples ran excellently, and separation was good (hexane: ethyl acetate) in a ratio of 8.5:1.5 v/v. Compared to the test drug with an active constituent, the RF valve is the same.\u003c/p\u003e\u003ch2\u003eResult\u003c/h2\u003e\u003cp\u003eIn antioxidant activity, antioxidant properties with DPPH and compare them to the standard antioxidant drug, Gallic acid. In this activity we prepare the different concentrations of the solution and check which concentration shows the high antioxidant activity and compare it with the standard drug concentration activity.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e\u003cp\u003eThe test drug antioxidant property is high in the 100 \u0026micro;L concentration. The sample is run in UV-visible spectroscopy at 517 nm, and the standard is run at 270nm for the absorbance of the drug.\u003c/p\u003e\u003ch2\u003eMajor Finding:\u003c/h2\u003e\u003cp\u003eThe study revealed that both \u003cem\u003ePsoralea corylifolia\u003c/em\u003e and \u003cem\u003eNigella sativa\u003c/em\u003e extracts exhibit strong antioxidant activity, with \u003cem\u003eNigella sativa\u003c/em\u003e showing slightly higher free radical scavenging potential. Spectroscopic analysis confirmed the presence of phenolic and flavonoid compounds contributing to their antioxidant properties.\u003c/p\u003e","manuscriptTitle":"Evaluation of Antioxidant and Spectroscopic analysis of Psoralea corylifolia and Nigella sativa Plant Extract","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-11-02 18:08:30","doi":"10.21203/rs.3.rs-7750071/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"a36d911d-d582-4449-956a-37e6076f2b05","owner":[],"postedDate":"November 2nd, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-11-25T09:09:06+00:00","versionOfRecord":[],"versionCreatedAt":"2025-11-02 18:08:30","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7750071","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7750071","identity":"rs-7750071","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}