Long-Term Maintenance of Viable Human Endometrial Epithelial Cells to Analyze Estrogen and Progestin Effects.

Riaz MA, Kary FL, Jensen A, Zeppernick F, Ivo Meinhold‐Heerlein, Lutz Konrad
Cells · 2024 · doi:10.3390/cells13100811 · PMID:38786035 · PMC11120542
OA: gold CC-BY-4.0
📄 Open PDF View on PubMed View at publisher

Abstract

There are fewer investigations conducted on human primary endometrial epithelial cells (HPEECs) compared to human primary endometrial stromal cells (HPESCs). One of the main reasons is the scarcity of protocols enabling prolonged epithelial cell culture. Even though it is possible to culture HPEECs in 3D over a longer period of time, it is technically demanding. In this study, we successfully established a highly pure, stable, and long-term viable human conditionally reprogrammed endometrial epithelial cell line, designated as eCRC560. These cells stained positive for epithelial markers, estrogen and progesterone receptors, and epithelial cell-cell contacts but negative for stromal and endothelial cell markers. Estradiol (ES) reduced the abundance of ZO-1 in a time- and dose-dependent manner, in contrast to the dose-dependent increase with the progestin dienogest (DNG) when co-cultured with HPESCs. Moreover, ES significantly increased cell viability, cell migration, and invasion of the eCRC560 cells; all these effects were inhibited by pretreatment with DNG. DNG withdrawal led to a significantly disrupted monolayer of eCRC560 cells in co-culture with HPESCs, yet it markedly increased the adhesion of eCRC560 to the human mesothelial MeT-5A cells. The long-term viable eCRC560 cells are suitable for in vitro analysis of HPEECs to study the epithelial compartment of the human endometrium and endometrial pathologies.

My notes (saved in your browser only)

Citation neighborhood (no data yet)

We don't have any in-corpus citations linked to this paper yet. This is a recent paper (2024) — citers typically take a year or two to land, and the OpenAlex reference graph may still be filling in.

SciLite annotations

organisms 36
human human human human human human transgenic mice transgenic mice human human human human human weinbergillus histolyticus somali wild ass rabbits transgenic mice somali wild ass rabbits rabbits transgenic mice rabbits rabbits transgenic mice human human human transgenic mice human human human human human human human human
chemicals 62
estrogen estradiol progestin dienogest estrogen progestin steroid y-27632 y-27632 y-27632 palmitoyl amino acid penicillin streptomycin water carbohydroximic acid glucose glutamine glutamine selenium l-glutamine photon water water water y-27632 water ethanol dienogest 6beta-[n-(carboxymethylamino)carbonyl]methoxy-17beta-estradiol methanol polysorbate 20 alexa fluor 488 phenylindole ethanol trypan blue calcein am 22:2-18:3-pc 22:2-18:3-pc y-27632 steroid hormone progestin progesterone estrogen progesterone estrogen progesterone estradiol estrogen polyacrylamide macromolecule progesterone progesterone progestin progestin progestin progestin progesterone progesterone progestin progestin steroid +2 more

Source provenance

europepmc
last seen: 2026-07-21T06:11:07.228698+00:00
scilite
last seen: 2026-05-18T04:57:49.680383+00:00
unpaywall
last seen: 2026-05-21T05:10:58.409756+00:00
License: CC-BY-4.0