Therapeutic Potential of GYY4137 in Reducing Oxidative

Therapeutic Potential of GYY4137 in Reducing Oxidative | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Therapeutic Potential of GYY4137 in Reducing Oxidative Lucile Daubresse, Marion Marlinge, Hélène Lavner, Julia-Sophie Dodivers, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7039867/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 05 Mar, 2026 Read the published version in Scientific Reports → Version 1 posted 12 You are reading this latest preprint version Abstract The pathophysiology of decompression sickness (DCS) is not fully understood. Apart from 13 bubble formation, endothelial dysfunction and reactive oxygen species (ROS) production, 14 participate in DCS. We aimed to evaluate the redox profile (redox potential, adenosine 15 deaminase activity (ADA), and xanthine oxidase activity (XO)) and the effects of GYY4137, an 16 H2S donor with antiradical properties, on the mortality of mice exposed to experimental DCS. 17 Sixty Mice were injected intraperitoneally with either GYY4137 or vehicle, then subjected to 18 high pressure in a hyperbaric chamber, followed by a quick decompression. GYY4137 increased 19 survival with a median lethal dose (LD50) of 120 m compared with those injected with vehicle (< 20 100 m; p = 0.038) but did not affect ADA or XO activities. The redox potential (RP: mV) was 21 lower in the GYY4137 group (116[78-189]) than that in the vehicle group: 150[88-226],p = 0.04. 22 Experimental decompression sickness itself is associated with an increase in redox potential. We 23 concluded that GYY4137 protects mice while reducing potential redox and ROS production. Our 24 results seem to indicate that the reduction of the redox potential induced by the administration 25 of thiol donor could reduce morbidity and mortality during DCS. Biological sciences/Biochemistry Health sciences/Diseases Biological sciences/Drug discovery Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 05 Mar, 2026 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 03 Nov, 2025 Reviewers agreed at journal 13 Sep, 2025 Reviews received at journal 11 Sep, 2025 Reviewers agreed at journal 28 Aug, 2025 Reviews received at journal 19 Jul, 2025 Reviewers agreed at journal 12 Jul, 2025 Reviewers agreed at journal 09 Jul, 2025 Reviewers invited by journal 09 Jul, 2025 Editor assigned by journal 09 Jul, 2025 Editor invited by journal 09 Jul, 2025 Submission checks completed at journal 07 Jul, 2025 First submitted to journal 03 Jul, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7039867","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":483001785,"identity":"3503f6ac-3593-40f3-b64f-8b0be97b1d1d","order_by":0,"name":"Lucile Daubresse","email":"","orcid":"","institution":"Institut de Recherche en Biologie du Service de santé des Armée (IRBA)s","correspondingAuthor":false,"prefix":"","firstName":"Lucile","middleName":"","lastName":"Daubresse","suffix":""},{"id":483001786,"identity":"cae5961f-4653-49f5-a28b-b7b8dfee91d0","order_by":1,"name":"Marion Marlinge","email":"","orcid":"","institution":"Aix Marseille University","correspondingAuthor":false,"prefix":"","firstName":"Marion","middleName":"","lastName":"Marlinge","suffix":""},{"id":483001787,"identity":"78a85a07-cbad-43e5-adfe-72a28e34fe14","order_by":2,"name":"Hélène Lavner","email":"","orcid":"","institution":"Assitance Publique des Hopitaux de Marseille (AP-HM)","correspondingAuthor":false,"prefix":"","firstName":"Hélène","middleName":"","lastName":"Lavner","suffix":""},{"id":483001788,"identity":"081389eb-bbd2-49c1-8058-bf3ddf478ecb","order_by":3,"name":"Julia-Sophie Dodivers","email":"","orcid":"","institution":"Aix Marseille University","correspondingAuthor":false,"prefix":"","firstName":"Julia-Sophie","middleName":"","lastName":"Dodivers","suffix":""},{"id":483001789,"identity":"311c1be8-ca1a-42d0-8128-2668929dcd17","order_by":4,"name":"Alexandrine Bertaud","email":"","orcid":"","institution":"Aix Marseille University","correspondingAuthor":false,"prefix":"","firstName":"Alexandrine","middleName":"","lastName":"Bertaud","suffix":""},{"id":483001790,"identity":"0b4d6bde-5cd2-4d56-9c2c-79df199a5ea0","order_by":5,"name":"Simon Lledo","email":"","orcid":"","institution":"Aix Marseille University","correspondingAuthor":false,"prefix":"","firstName":"Simon","middleName":"","lastName":"Lledo","suffix":""},{"id":483001791,"identity":"ce2dec36-1d53-4ab0-a964-fe6b83a4a0fe","order_by":6,"name":"Samantha Conte","email":"","orcid":"","institution":"Aix Marseille University","correspondingAuthor":false,"prefix":"","firstName":"Samantha","middleName":"","lastName":"Conte","suffix":""},{"id":483001792,"identity":"49f37bce-0006-4f46-8c0d-f2510f465b0a","order_by":7,"name":"Julien Fromonot","email":"","orcid":"","institution":"Aix Marseille University","correspondingAuthor":false,"prefix":"","firstName":"Julien","middleName":"","lastName":"Fromonot","suffix":""},{"id":483001793,"identity":"ac64d9d4-ece4-45a0-b205-0ab0735d4c73","order_by":8,"name":"Marguerite Gastaldi","email":"","orcid":"","institution":"Assitance Publique des Hopitaux de Marseille (AP-HM)","correspondingAuthor":false,"prefix":"","firstName":"Marguerite","middleName":"","lastName":"Gastaldi","suffix":""},{"id":483001794,"identity":"e4654662-22f0-4f53-bf18-bf6573763d4a","order_by":9,"name":"Jean Jacques Risso","email":"","orcid":"","institution":"Institut de Recherche en Biologie du Service de santé des Armée (IRBA)s","correspondingAuthor":false,"prefix":"","firstName":"Jean","middleName":"Jacques","lastName":"Risso","suffix":""},{"id":483001795,"identity":"713bc5ff-d220-44d8-9797-d14ebe37c636","order_by":10,"name":"Jean Claude Rostain","email":"","orcid":"","institution":"Aix Marseille University","correspondingAuthor":false,"prefix":"","firstName":"Jean","middleName":"Claude","lastName":"Rostain","suffix":""},{"id":483001796,"identity":"a28266b2-c04d-4f10-a9b8-44bb3adb4899","order_by":11,"name":"Jean Eric Blatteau","email":"","orcid":"","institution":"Institut de Recherche en Biologie du Service de santé des Armée (IRBA)s","correspondingAuthor":false,"prefix":"","firstName":"Jean","middleName":"Eric","lastName":"Blatteau","suffix":""},{"id":483001797,"identity":"78f5d955-4b2e-40bd-a2de-9bc08538518d","order_by":12,"name":"Nicolas Vallee","email":"","orcid":"","institution":"Institut de Recherche en Biologie du Service de santé des Armée (IRBA)s","correspondingAuthor":false,"prefix":"","firstName":"Nicolas","middleName":"","lastName":"Vallee","suffix":""},{"id":483001798,"identity":"d3c8a782-9e46-4935-8f14-32fbce5d39ff","order_by":13,"name":"Régis Guieu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA70lEQVRIiWNgGAWjYFACxgYIzczA+AAm9oFYLcwGMKEZxNrHJkGUFvmI5MbHPAz35OTd2Z9VfNxjlyfv3sDYXIFHi+GNxGZjHoZiY8PDPGY3ZzxLLjY8c4Cx8Qw+LTMS26R5GBISNzbzsN3mOcCcuHFGAvvDBvxa2n8DtdRvbGZ/VsxzoD5x4/wHjI34tMhLJLYxA7UkyDMzmDHzHDicOF+CAb8WA56HzZJzDBIMNzDzGEvOOHA8cQNPYiN+W9rTH354U5EgL99//OGHDweqE+e3Hz6I35YDYBLGAIvAIheXLQ04GaNgFIyCUTAKoAAA8e9Ouexl4S4AAAAASUVORK5CYII=","orcid":"","institution":"Aix Marseille University","correspondingAuthor":true,"prefix":"","firstName":"Régis","middleName":"","lastName":"Guieu","suffix":""}],"badges":[],"createdAt":"2025-07-03 15:53:06","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7039867/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7039867/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41598-026-41352-2","type":"published","date":"2026-03-05T15:58:40+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":104251806,"identity":"4a00921b-7c76-40ed-9330-bc5d61d1bafa","added_by":"auto","created_at":"2026-03-09 16:15:25","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":524178,"visible":true,"origin":"","legend":"","description":"","filename":"MainBiodydef.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7039867/v1_covered_7ed61229-0017-47d2-8390-fa08af874a17.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Therapeutic Potential of GYY4137 in Reducing Oxidative","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-7039867/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7039867/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"The pathophysiology of decompression sickness (DCS) is not fully understood. Apart from 13 bubble formation, endothelial dysfunction and reactive oxygen species (ROS) production, 14 participate in DCS. We aimed to evaluate the redox profile (redox potential, adenosine 15 deaminase activity (ADA), and xanthine oxidase activity (XO)) and the effects of GYY4137, an 16 H2S donor with antiradical properties, on the mortality of mice exposed to experimental DCS. 17 Sixty Mice were injected intraperitoneally with either GYY4137 or vehicle, then subjected to 18 high pressure in a hyperbaric chamber, followed by a quick decompression. GYY4137 increased 19 survival with a median lethal dose (LD50) of 120 m compared with those injected with vehicle (\u003c 20 100 m; p = 0.038) but did not affect ADA or XO activities. The redox potential (RP: mV) was 21 lower in the GYY4137 group (116[78-189]) than that in the vehicle group: 150[88-226],p = 0.04. 22 Experimental decompression sickness itself is associated with an increase in redox potential. We 23 concluded that GYY4137 protects mice while reducing potential redox and ROS production. Our 24 results seem to indicate that the reduction of the redox potential induced by the administration 25 of thiol donor could reduce morbidity and mortality during DCS.","manuscriptTitle":"Therapeutic Potential of GYY4137 in Reducing Oxidative","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-07-10 17:33:41","doi":"10.21203/rs.3.rs-7039867/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-11-03T08:36:58+00:00","index":"","fulltext":""},{"type":"reviewerAgreed","content":"130890790412316812396268919925749142080","date":"2025-09-13T13:21:37+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-09-11T09:53:36+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"141811671762548049096389111458750573725","date":"2025-08-28T10:38:46+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-07-19T23:44:51+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"125429904919153974328716557504727111020","date":"2025-07-12T09:44:02+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"311468822854852614549463490959688352711","date":"2025-07-09T13:56:10+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-07-09T06:37:03+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-07-09T06:35:54+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2025-07-09T06:14:23+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-07-08T03:08:38+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2025-07-03T15:38:12+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"e381b13a-56d7-4c52-b046-6de399bcc3c9","owner":[],"postedDate":"July 10th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":51286623,"name":"Biological sciences/Biochemistry"},{"id":51286624,"name":"Health sciences/Diseases"},{"id":51286625,"name":"Biological sciences/Drug discovery"}],"tags":[],"updatedAt":"2026-03-09T16:10:36+00:00","versionOfRecord":{"articleIdentity":"rs-7039867","link":"https://doi.org/10.1038/s41598-026-41352-2","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2026-03-05 15:58:40","publishedOnDateReadable":"March 5th, 2026"},"versionCreatedAt":"2025-07-10 17:33:41","video":"","vorDoi":"10.1038/s41598-026-41352-2","vorDoiUrl":"https://doi.org/10.1038/s41598-026-41352-2","workflowStages":[]},"version":"v1","identity":"rs-7039867","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7039867","identity":"rs-7039867","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}