Bisdemethoxycurcumin attenuates sciatic nerve damage in Vincristine- induced Neuropathic Pain Mice

Bisdemethoxycurcumin attenuates sciatic nerve damage in Vincristine- induced Neuropathic Pain Mice | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Bisdemethoxycurcumin attenuates sciatic nerve damage in Vincristine- induced Neuropathic Pain Mice Chalton Manengu This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9700117/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Chemotherapy-induced neuropathic pain is a common side effect for cancer patients. There are many therapeutic choices available today, but they typically have negative side effects and are ineffective at reducing pain. As a result, novel strategies for treating chemotherapy induced neuropathic pain are being investigated, particularly those involving natural substances. Bisdemethoxycurcumin (BDCM), a component of turmeric, is a potential target. This study explores the potential of BDCM in lessening pain sensitivity and restoring sciatic nerve damage. Methodology Mice were injected with vincristine sulfate intraperitoneally to create the study model. The cold plate test, thermal radiation test, and Von Frey test were used to evaluate the potential pain-relieving benefits of BDCM. We employed transmission electron microscopy (TEM), hematoxylin and eosin staining, silver staining, and an electrophysiological stimulation test to assess BDCM's possible neuroprotective effect on the sciatic nerve. We further evaluated BDCM’s effects on NF-κB and pro-inflammatory cytokines. Results BDCM treatment reduced the frequency of paw lifts, increased the latency before paw withdrawal, and significantly raised the paw withdrawal threshold. BDCM reduced sciatic nerve tissue damage and increased sensory nerve conduction velocity. Additionally, BDCM decreased NF-κB and pro-inflammatory cytokines expression. Conclusion These findings demonstrate that BDCM alleviates VCR-induced sciatic nerve damage and preserves nerve integrity, highlighting its potential as a novel therapeutic agent for neuropathic pain and further studies should further explore its mechanisms in neuropathic pain attenuation. Clinical Pharmacology Bisdemethoxycurcumin vincristine chemotherapy neuropathic pain antinociception neuroprotection Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9700117","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":639525460,"identity":"b286f7af-6f4e-4dac-8fa1-391b0820f9b0","order_by":0,"name":"Chalton Manengu","email":"data:image/png;base64,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","orcid":"","institution":"Ningxia Medical University","correspondingAuthor":true,"prefix":"","firstName":"Chalton","middleName":"","lastName":"Manengu","suffix":""}],"badges":[],"createdAt":"2026-05-13 07:38:18","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-9700117/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-9700117/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":109295996,"identity":"df681414-431a-45be-b5e9-e4169fdad62b","added_by":"auto","created_at":"2026-05-15 08:43:25","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1012656,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-9700117/v1_covered_ce59570d-1f50-4723-ba85-175c78a5d958.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eBisdemethoxycurcumin attenuates sciatic nerve damage in Vincristine- induced Neuropathic Pain Mice\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"Ningxia Medical University","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Bisdemethoxycurcumin, vincristine, chemotherapy, neuropathic pain, antinociception, neuroprotection","lastPublishedDoi":"10.21203/rs.3.rs-9700117/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9700117/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eChemotherapy-induced neuropathic pain is a common side effect for cancer patients. 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We employed transmission electron microscopy (TEM), hematoxylin and eosin staining, silver staining, and an electrophysiological stimulation test to assess BDCM's possible neuroprotective effect on the sciatic nerve. We further evaluated BDCM’s effects on NF-κB and pro-inflammatory cytokines.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eBDCM treatment reduced the frequency of paw lifts, increased the latency before paw withdrawal, and significantly raised the paw withdrawal threshold. BDCM reduced sciatic nerve tissue damage and increased sensory nerve conduction velocity. 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