Characterization of a Bacteriophage Isolated from a River water against a Local Field Strain Multi-Drug Resistant Staphylococcus Aureus

Abstract It is becoming increasingly difficult in combating Multi-drug resistant (MDR) bacteria. MDR Staphylococcus aureus particularly methicillin-resistant S. aureus is one such notorious pathogen in clinical settings and the food industry. With increasing incidences of drug resistance and slow progress in developing new antibiotics, bacteriophages against pathogenic S. aureus are promising as antibacterial. We isolated Four local field MDR S. aureus from wastewater samples. We got a bacteriophage against an MDR S. aureus from a river-water sample. The bacteriophage was lytic and was stable at various temperatures ranging from − 20°C to 37°C. the bacteriophage was stable at a highly alkaline PH and had a narrow host range. Through genomic analysis, the bacteriophage DNA encodes 52 genes, and all predicted genes are on one strand, it also encodes a phage RNA polymerase; although it does not show similarity to any known staphylococcal bacteriophage, it shows similarity (91%) to Enterobacteriaceae phages. When surveying the research articles about Staphylococcal phages, we could find about the unclassified and Singleton-Staphylococcal phages.