Genome-wide identification and expression analysis of kinesin genes superfamily suggested their roles in response to abiotic stress and fertility of wheat (Triticum aestivum L.)

Genome-wide identification and expression analysis of kinesin genes superfamily suggested their roles in response to abiotic stress and fertility of wheat (Triticum aestivum L.) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Genome-wide identification and expression analysis of kinesin genes superfamily suggested their roles in response to abiotic stress and fertility of wheat (Triticum aestivum L.) Qinge Chen, Yang Ren, Qin Yan, Zhiyuan Zheng, Gaisheng Zhang, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4642123/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 19 Dec, 2024 Read the published version in BMC Genomics → Version 1 posted 4 You are reading this latest preprint version Abstract Background Kinesin is a motor for microtubule-based motility and plays a vital role in plant growth and development. The kinesins superfamily members are mainly known from Arabidopsis. However, little research about kinesins superfamily has been conducted on hexploid wheat ( Triticum aestivum L.). The functions of kinesins in wheat growth and development, regulation of cell division and response to stress are still unclear. Results In this study, we identified 155 TaKIN genes in wheat, which were divided into 10 families and some ungrouped proteins by phylogenetic analysis. Less gene structural differences showed that TaKIN genes had redundant functions. The conserved domains of different family members were different, and some families might have some special functional domains. We found many cis-acting elements related to hormones (GA, Auxin, SA, MeJA), cell cycle, cell division in homeopathic elements of TaKIN genes. Collinearity analysis showed that TaKIN genes were more conservative in monocotyledons. Expression level in different tissues at different stages suggested that TaKIN family may function during the whole growth and development process of wheat. It was worth noting there were quite different at gene expression level between physiological and heritable male sterile lines during the different stages of pollen development. The differential expression patterns of some TaKIN genes between male sterile line and maintainer line might be related to wheat male sterile. Furthermore, we also found TaKIN genes were involved in response to plant hormones and abiotic stress by stress assays. Conclusions The result is useful for further exploring the molecular mechanism of kinesin genes in wheat male sterility and provides the important information in response to plant hormones and abiotic stress. Wheat Kinesin Expression Male sterile Hormone response Full Text Additional Declarations No competing interests reported. Supplementary Files Fig.S1.tif Fig. S1. The expression pattern analysis of TaKIN gene in leaf of wheat. See: seedling stage; 3-lea: three leaf stage; 5-lea: fifth leaf stage; Till: tillering stage; F-lea: Flag leaf stage; Fboot: Full boot; 30%sp: 30% spike; Emer: Ear emergence; Mil-g: milk grain stage.1-lb: first leaf blade; 3-lb: third leaf blade; 5-lb: fifth leaf blade; Flb: flag leaf blade. Fig.S2.tif Fig. S2. The expression pattern analysis of TaKIN gene in root of wheat. See: seedling stage; 1-lea: first leaf blade; 3-lea: three leaf stage; 5-lea: fifth leaf stage; Till: tillering stage; F-lea: Flag leaf stage. Fig.S3.tif Fig. S3. The expression pattern analysis of TaKIN gene in grain of wheat. Mil-g: milk grain stage; S-dou: soft dough; D-dou: hard dough;. Fig.S4.tif Fig. S4. The expression pattern analysis of TaKIN gene in spikes of wheat. Fboot: Full boot; 30%sp: 30% spike; Emer: Ear emergence; Anth: anthesis; Mil-g: milk-ripe stage; Spike: spike; s-let: spikelets; awn: awns; glu: glumes; anth: anther; st-ov: stigma & ovary; lem: lemma. suplementarytables.docx Table S1. Identification of kinesin genes in wheat, physicochemical characteristics of proteins, and gene duplication patterns. Table S2 The subgenome distribution of Kinesin homologs in wheat. Table S3 sequence of qRT-PCR primers. Cite Share Download PDF Status: Published Journal Publication published 19 Dec, 2024 Read the published version in BMC Genomics → Version 1 posted Editorial decision: Revision requested 01 Jul, 2024 Editor assigned by journal 27 Jun, 2024 Submission checks completed at journal 27 Jun, 2024 First submitted to journal 26 Jun, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4642123","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":321407723,"identity":"68e95957-139c-47b7-a474-977405a0e92c","order_by":0,"name":"Qinge Chen","email":"","orcid":"","institution":"Northwest A\u0026F University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Qinge","middleName":"","lastName":"Chen","suffix":""},{"id":321407724,"identity":"e2d6fbc1-c3fe-452a-b139-a878e15f04c2","order_by":1,"name":"Yang Ren","email":"","orcid":"","institution":"Northwest A\u0026F University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Yang","middleName":"","lastName":"Ren","suffix":""},{"id":321407726,"identity":"9ee51d21-1e23-4daf-9349-fdf384a77d19","order_by":2,"name":"Qin Yan","email":"","orcid":"","institution":"Northwest A\u0026F University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Qin","middleName":"","lastName":"Yan","suffix":""},{"id":321407728,"identity":"a27eabb8-72b0-4232-b76d-64797c6d56e2","order_by":3,"name":"Zhiyuan Zheng","email":"","orcid":"","institution":"Northwest A\u0026F University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Zhiyuan","middleName":"","lastName":"Zheng","suffix":""},{"id":321407730,"identity":"c0a3e9dc-fee1-4c71-8c20-748ae4eeb8d6","order_by":4,"name":"Gaisheng Zhang","email":"","orcid":"","institution":"Northwest A\u0026F University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Gaisheng","middleName":"","lastName":"Zhang","suffix":""},{"id":321407732,"identity":"7f1a436f-440d-4921-8623-b780d74d7157","order_by":5,"name":"Lingjian Ma","email":"","orcid":"","institution":"Northwest A\u0026F University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Lingjian","middleName":"","lastName":"Ma","suffix":""},{"id":321407734,"identity":"60bf4e05-e1fa-41a4-a36a-1d4ae097ebbf","order_by":6,"name":"Qilu Song","email":"","orcid":"","institution":"Peking University Institute of Advanced Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"Qilu","middleName":"","lastName":"Song","suffix":""},{"id":321407735,"identity":"346e6baa-7055-4a87-9816-c80234848a59","order_by":7,"name":"Na Niu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAzklEQVRIiWNgGAWjYDACZhBRAWF/gHKJ0XIGzGScQZwWsNo2UrSYs/OYPfg5707i/BnJDxsYKqwTG9jPHsCrxbKZx9ywd9uzxA030gwbGM6kJzbw5CXg1WJwmMdMgnfb4dwNEgnmDxjbDic2SPAYENQi+XfO4dz5M9I/NjD+I1KLNG/D4dyGGzmGDYwNRGlhK5OWOXa4fsOZN4UNCcfSjdt4cghoOX94m+SbmsPG8u3pGxs+1FjL9rOfwa8FFSQAMRsJ6kfBKBgFo2AU4AAA0gNGj2HxuD8AAAAASUVORK5CYII=","orcid":"","institution":"Northwest A\u0026F University of Agriculture","correspondingAuthor":true,"prefix":"","firstName":"Na","middleName":"","lastName":"Niu","suffix":""}],"badges":[],"createdAt":"2024-06-26 10:51:05","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4642123/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4642123/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1186/s12864-024-11156-7","type":"published","date":"2024-12-19T15:58:24+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":72202670,"identity":"75b4e01e-a4d4-4868-ac01-a37cf2fc1320","added_by":"auto","created_at":"2024-12-23 16:15:30","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1756472,"visible":true,"origin":"","legend":"","description":"","filename":"MbBMCGenomics.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4642123/v1_covered_d1e04aeb-11b1-450f-a907-991f1a97e4c4.pdf"},{"id":60684118,"identity":"68b43e07-6f2c-4c63-bf15-1e05e60381d9","added_by":"auto","created_at":"2024-07-19 13:18:18","extension":"tif","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":3323728,"visible":true,"origin":"","legend":"\u003cp\u003eFig. S1. The expression pattern analysis of \u003cem\u003eTaKIN\u003c/em\u003egene in leaf of wheat. See: seedling stage; 3-lea: three leaf stage; 5-lea: fifth leaf stage; Till: tillering stage; F-lea: Flag leaf stage; Fboot: Full boot; 30%sp: 30% spike; Emer: Ear emergence; Mil-g: milk grain stage.1-lb: first leaf blade; 3-lb: third leaf blade; 5-lb: fifth leaf blade; Flb: flag leaf blade.\u003c/p\u003e","description":"","filename":"Fig.S1.tif","url":"https://assets-eu.researchsquare.com/files/rs-4642123/v1/013dc3ad8aad52c73eeb1731.tif"},{"id":60682889,"identity":"3adad8f5-eb23-4ba4-bd18-61b4ea542273","added_by":"auto","created_at":"2024-07-19 13:02:17","extension":"tif","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":2063780,"visible":true,"origin":"","legend":"\u003cp\u003eFig. S2. The expression pattern analysis of \u003cem\u003eTaKIN\u003c/em\u003egene in root of wheat. See: seedling stage; 1-lea: first leaf blade; 3-lea: three leaf stage; 5-lea: fifth leaf stage; Till: tillering stage; F-lea: Flag leaf stage.\u003c/p\u003e","description":"","filename":"Fig.S2.tif","url":"https://assets-eu.researchsquare.com/files/rs-4642123/v1/871d3440e75185b78017184c.tif"},{"id":60682891,"identity":"22d2b86c-7775-4631-b7e9-f2a896d3bcb6","added_by":"auto","created_at":"2024-07-19 13:02:17","extension":"tif","order_by":3,"title":"","display":"","copyAsset":false,"role":"supplement","size":1579076,"visible":true,"origin":"","legend":"\u003cp\u003eFig. S3. The expression pattern analysis of \u003cem\u003eTaKIN\u003c/em\u003egene in grain of wheat. Mil-g: milk grain stage; S-dou: soft dough; D-dou: hard dough;.\u003c/p\u003e","description":"","filename":"Fig.S3.tif","url":"https://assets-eu.researchsquare.com/files/rs-4642123/v1/c726c26a0fd4112766f2b6b4.tif"},{"id":60682892,"identity":"0f4a1793-3ae3-416a-9fcf-0a3ed36f14c9","added_by":"auto","created_at":"2024-07-19 13:02:17","extension":"tif","order_by":4,"title":"","display":"","copyAsset":false,"role":"supplement","size":3240384,"visible":true,"origin":"","legend":"\u003cp\u003e1. Fig. S4. The expression pattern analysis of \u003cem\u003eTaKIN\u003c/em\u003egene in spikes of wheat. Fboot: Full boot; 30%sp: 30% spike; Emer: Ear emergence; Anth: anthesis; Mil-g: milk-ripe stage; Spike: spike; s-let: spikelets; awn: awns; glu: glumes; anth: anther; st-ov: stigma \u0026amp; ovary; lem: lemma.\u003c/p\u003e","description":"","filename":"Fig.S4.tif","url":"https://assets-eu.researchsquare.com/files/rs-4642123/v1/f85c31b5933f8a315c71e224.tif"},{"id":60683668,"identity":"62e69abf-fd4f-47ea-963f-61e321dcb155","added_by":"auto","created_at":"2024-07-19 13:10:17","extension":"docx","order_by":5,"title":"","display":"","copyAsset":false,"role":"supplement","size":32938,"visible":true,"origin":"","legend":"\u003cp\u003e1. Table S1. Identification of kinesin genes in wheat, physicochemical characteristics of proteins, and gene duplication patterns.\u003c/p\u003e\n\u003cp\u003e2. Table S2 The subgenome distribution of Kinesin homologs in wheat.\u003c/p\u003e\n\u003cp\u003e3. Table S3 sequence of qRT-PCR primers.\u003c/p\u003e","description":"","filename":"suplementarytables.docx","url":"https://assets-eu.researchsquare.com/files/rs-4642123/v1/880028084534c3883e4d4227.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Genome-wide identification and expression analysis of kinesin genes superfamily suggested their roles in response to abiotic stress and fertility of wheat (Triticum aestivum L.)","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"bmc-genomics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"gics","sideBox":"Learn more about [BMC Genomics](http://bmcgenomics.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/gics","title":"BMC Genomics","twitterHandle":"#BMCGenomics","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Wheat, Kinesin, Expression, Male sterile, Hormone response","lastPublishedDoi":"10.21203/rs.3.rs-4642123/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4642123/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eKinesin is a motor for microtubule-based motility and plays a vital role in plant growth and development. The kinesins superfamily members are mainly known from Arabidopsis. However, little research about kinesins superfamily has been conducted on hexploid wheat (\u003cem\u003eTriticum aestivum\u003c/em\u003e L.). The functions of kinesins in wheat growth and development, regulation of cell division and response to stress are still unclear.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eIn this study, we identified 155 \u003cem\u003eTaKIN\u003c/em\u003e genes in wheat, which were divided into 10 families and some ungrouped proteins by phylogenetic analysis. Less gene structural differences showed that \u003cem\u003eTaKIN\u003c/em\u003e genes had redundant functions. The conserved domains of different family members were different, and some families might have some special functional domains. We found many cis-acting elements related to hormones (GA, Auxin, SA, MeJA), cell cycle, cell division in homeopathic elements of \u003cem\u003eTaKIN\u003c/em\u003e genes. Collinearity analysis showed that \u003cem\u003eTaKIN\u003c/em\u003e genes were more conservative in monocotyledons. Expression level in different tissues at different stages suggested that TaKIN family may function during the whole growth and development process of wheat. It was worth noting there were quite different at gene expression level between physiological and heritable male sterile lines during the different stages of pollen development. The differential expression patterns of some \u003cem\u003eTaKIN\u003c/em\u003e genes between male sterile line and maintainer line might be related to wheat male sterile. Furthermore, we also found \u003cem\u003eTaKIN\u003c/em\u003e genes were involved in response to plant hormones and abiotic stress by stress assays.\u003c/p\u003e\u003ch2\u003eConclusions\u003c/h2\u003e \u003cp\u003eThe result is useful for further exploring the molecular mechanism of kinesin genes in wheat male sterility and provides the important information in response to plant hormones and abiotic stress.\u003c/p\u003e","manuscriptTitle":"Genome-wide identification and expression analysis of kinesin genes superfamily suggested their roles in response to abiotic stress and fertility of wheat (Triticum aestivum L.)","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-07-19 13:02:12","doi":"10.21203/rs.3.rs-4642123/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-07-01T21:26:01+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-06-28T00:44:04+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-06-28T00:43:45+00:00","index":"","fulltext":""},{"type":"submitted","content":"BMC Genomics","date":"2024-06-26T10:49:41+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-genomics","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"gics","sideBox":"Learn more about [BMC Genomics](http://bmcgenomics.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/gics","title":"BMC Genomics","twitterHandle":"#BMCGenomics","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"4d561b97-1b1e-4fa5-a79d-c90701101873","owner":[],"postedDate":"July 19th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2024-12-23T16:09:13+00:00","versionOfRecord":{"articleIdentity":"rs-4642123","link":"https://doi.org/10.1186/s12864-024-11156-7","journal":{"identity":"bmc-genomics","isVorOnly":false,"title":"BMC Genomics"},"publishedOn":"2024-12-19 15:58:24","publishedOnDateReadable":"December 19th, 2024"},"versionCreatedAt":"2024-07-19 13:02:12","video":"","vorDoi":"10.1186/s12864-024-11156-7","vorDoiUrl":"https://doi.org/10.1186/s12864-024-11156-7","workflowStages":[]},"version":"v1","identity":"rs-4642123","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4642123","identity":"rs-4642123","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}