Fungal Endophytes Associated With the Actinorhizal Plant Species, Elaeagnus Latifolia L. (Elaeagnaceae) And Evaluating Their Antagonistic Potential Against Grey Blight Disease In Tea [Camellia Sinensis (L.) O. Kuntze]: A Novel Study From North-East India

Fungal Endophytes Associated With the Actinorhizal Plant Species, Elaeagnus Latifolia L. (Elaeagnaceae) And Evaluating Their Antagonistic Potential Against Grey Blight Disease In Tea [Camellia Sinensis (L.) O. Kuntze]: A Novel Study From North-East India | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Fungal Endophytes Associated With the Actinorhizal Plant Species, Elaeagnus Latifolia L. ( Elaeagnaceae ) And Evaluating Their Antagonistic Potential Against Grey Blight Disease In Tea [ Camellia Sinensis (L.) O. Kuntze]: A Novel Study From North-East India P. N. Bhattacharyya, L. H. Bhattacharyya, V. Parkash This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-260294/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The present investigation is a novel approach of exploring the endophytic fungal diversity of Elaeagnus latifolia L., an actinorhizal plant species of North-east India and evaluation of its biocontrol potential against Pestalotiopsis theae , the causal agent of grey blight disease in tea. A total of 17 endophytic fungal species belonging to 12 families and 03 orders were isolated from various parts of E. latifolia L. Isolates were identified based on colony morphology, spore and fruiting bodies using microscopical tools and techniques. Nigrospora sp. showed highest species density (0.5) among all the isolates. Isolation frequency was maximum (67%) in case of Fusarium sp., Nigrospora sp., Penicillium chrysogenum and Rhizopus sp. More fungal isolates were obtained from root and stem (47% each) as compared to leaves (29%). Species richness and diversity indices was maximum (15.0) in stem and minimum (9.0) in leaves. Highest Shannon and Simpson diversity index was in stem (2.02 and 0.860 respectively) followed by root (1.979 and 0.847 respectively) and least in leaves (1.494 and 0.75 respectively). Among the isolates tested for plant growth promoting parameters, EF09 ( Fusarium sp.) showed positive response for all the tested parameters. The isolate, EF09 also showed maximum antifungal potential (up to 87.1%) against P. theae in poisoned food technique. Enumeration of endophytic fungal diversity of E. latifolia L. holds promises as the putative strains may lead to the isolation of novel bioactive components for use in industry, medicine and agriculture. Applied & Industrial Microbiology Biotechnology and Bioengineering Actinorhizal plant species Antifungal potential Endophytes Elaeagnus latifolia L. Shannon and Simpson index value Poisoned food technique. Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Full Text Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the latest manuscript can be downloaded and accessed as a PDF. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-260294","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":14022825,"identity":"6ed37065-14c5-485b-808c-a509bc51fa9e","order_by":0,"name":"P. N. Bhattacharyya","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA/UlEQVRIiWNgGAWjYLACxoYDCWBGgoENiNt4gDgtbMwMDB8K0iBcorUwzvhwGCyAV4u59OFnD37uuJPHP7//4Gceg/N2a9sPA22psYnGpcWyL83csPfMs2KJY8zM0jwGt5O3nUkEajmWltuAQ4vBGQYzCd62w4kNx5gZwFrMDgC1MDYcxqOF/ZvkX6CW+UBbfvMYnEs2O/+QkBYeM2mQLRuOMbNJzjA4YGd2g4Atlj08ZdKyZ54lbjyWbGbxwSA5wewG0JYEPH4x52HfJvl2x53EeYcPPr6R8MfO3ux8+sMHH2pscDsMXSARrDIBh3KsWuzxKB4Fo2AUjIIRCgAFqmn9UNlsDAAAAABJRU5ErkJggg==","orcid":"https://orcid.org/0000-0002-6955-3696","institution":"Tocklai Tea Research Institute","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"P.","middleName":"N.","lastName":"Bhattacharyya","suffix":""},{"id":14022826,"identity":"1dd26154-aa62-4c6a-8cb3-e367a4365806","order_by":1,"name":"L. H. 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This map has been provided by the authors.","description":"","filename":"Onlinefloatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1/1d37d740bda7e5c60fcc9b61.png"},{"id":6539404,"identity":"bf510fc6-618f-4c40-84a0-8f2dca424332","added_by":"auto","created_at":"2021-03-02 21:13:08","extension":"jpeg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":188478,"visible":true,"origin":"","legend":"Collection of the plant specimen, Elaeagnus latifolia L. for enumeration of endophytic fungal assemblances.\n\n","description":"","filename":"floatimage2.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1/1a97edb04aa39e6f44283d04.jpeg"},{"id":6538924,"identity":"fa685596-94bf-4646-b5b2-1c51edaa8138","added_by":"auto","created_at":"2021-03-02 21:10:08","extension":"jpeg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":69479,"visible":true,"origin":"","legend":"Field identification of P. theae and culture growth pattern on PDA plates.","description":"","filename":"floatimage3.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1/c02c5088c25235472957c852.jpeg"},{"id":6538931,"identity":"afa03161-0644-42cb-af98-cc25e2ea9a54","added_by":"auto","created_at":"2021-03-02 21:10:08","extension":"jpeg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":81758,"visible":true,"origin":"","legend":"(A-L) Isolation of endophytic microflora from different plant parts of E. latifolia L.","description":"","filename":"floatimage4.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1/cde7ce899f4e3dba0c8731e7.jpeg"},{"id":6539405,"identity":"9979a00f-ae5c-4e06-bd8a-e4f23b6ef0bf","added_by":"auto","created_at":"2021-03-02 21:13:08","extension":"jpeg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":61486,"visible":true,"origin":"","legend":"(a-i) Pure culture plates of certain isolated endophytic strains.","description":"","filename":"floatimage5.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1/fdf9416c4fbceee1fc37c71a.jpeg"},{"id":6538928,"identity":"f4f095d7-7f5a-4c3c-9a02-a4f36502e5c3","added_by":"auto","created_at":"2021-03-02 21:10:08","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":24377,"visible":true,"origin":"","legend":"Density of fungal endophytes isolated from different plant parts of E. latifolia L.","description":"","filename":"Onlinefloatimage6.png","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1/eca48a81c15b495d75aeb884.png"},{"id":6539797,"identity":"7c980f76-a4d0-4adb-8a66-8231a307628e","added_by":"auto","created_at":"2021-03-02 21:16:08","extension":"jpeg","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":24454,"visible":true,"origin":"","legend":"Per cent occurrence of fungal endophytes in E. latifolia L..","description":"","filename":"floatimage7.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1/50c7df952f0f14558d760521.jpeg"},{"id":6538930,"identity":"4db53af1-4762-4ae2-9e58-eccfe1523391","added_by":"auto","created_at":"2021-03-02 21:10:08","extension":"jpeg","order_by":8,"title":"Figure 8","display":"","copyAsset":false,"role":"figure","size":59762,"visible":true,"origin":"","legend":"(A-D) Morphology of the antagonistic endophytic isolates on PDA media. A; endophytic microbial culture on PDA tubes; B. broth preparation for antagonistic screening; C. EF09, culture character; D. Zinc solubilisation potential by EF09.","description":"","filename":"floatimage8.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1/4e782944da2ecf97acfedb2e.jpeg"},{"id":6539407,"identity":"a7e7a754-b502-4459-8740-29c0db056dc7","added_by":"auto","created_at":"2021-03-02 21:13:08","extension":"jpeg","order_by":9,"title":"Figure 9","display":"","copyAsset":false,"role":"figure","size":26608,"visible":true,"origin":"","legend":"Antimicrobial evaluation of EF09 (Fusarium sp.) against P. theae using poisoned food method.","description":"","filename":"floatimage9.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1/fa7219d79d628843eddf30e6.jpeg"},{"id":13598926,"identity":"f69399fd-3c33-4d21-9bcc-178e65020537","added_by":"auto","created_at":"2021-09-17 05:37:38","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1468588,"visible":true,"origin":"","legend":"","description":"","filename":"Mainmanuscript19.02.2021.pdf","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1_covered.pdf"},{"id":11231255,"identity":"ea61e255-5aac-48fd-ae0b-afc0b9398394","added_by":"auto","created_at":"2021-07-07 22:19:07","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1465044,"visible":true,"origin":"","legend":"","description":"","filename":"Mainmanuscript19.02.2021.pdf","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1_covered.pdf"},{"id":6540038,"identity":"0832335e-ba94-49c4-bbfc-7839bf578d38","added_by":"auto","created_at":"2021-03-02 21:19:13","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1281116,"visible":true,"origin":"","legend":"","description":"","filename":"Mainmanuscript19.02.2021.pdf","url":"https://assets-eu.researchsquare.com/files/rs-260294/v1_stamped.pdf"}],"financialInterests":"","formattedTitle":"\u003cp\u003eFungal Endophytes Associated With the Actinorhizal Plant Species, \u003cem\u003eElaeagnus Latifolia\u003c/em\u003e L. (\u003cem\u003eElaeagnaceae\u003c/em\u003e) And Evaluating Their Antagonistic Potential Against Grey Blight Disease In Tea [\u003cem\u003eCamellia Sinensis \u003c/em\u003e(L.) O. Kuntze]: A Novel Study From North-East India\u003c/p\u003e","fulltext":[{"header":"Full Text","content":"Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the latest manuscript can be downloaded and \u003ca href='/article/rs-260294/latest.pdf' target='_blank'\u003e accessed as a PDF.\u003c/a\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Actinorhizal plant species, Antifungal potential, Endophytes, Elaeagnus latifolia L., Shannon and Simpson index value, Poisoned food technique.","lastPublishedDoi":"10.21203/rs.3.rs-260294/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-260294/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe present investigation is a novel approach of exploring the endophytic fungal diversity of \u003cem\u003eElaeagnus latifolia \u003c/em\u003eL., an actinorhizal plant species of North-east India and evaluation of its biocontrol potential against \u003cem\u003ePestalotiopsis theae\u003c/em\u003e, the causal agent of grey blight disease in tea. A total of 17 endophytic fungal species belonging to 12 families and 03 orders were isolated from various parts of\u003cem\u003e E. latifolia \u003c/em\u003eL.\u0026nbsp;Isolates were identified based on colony morphology, spore and fruiting bodies using microscopical tools and techniques. \u003cem\u003eNigrospora \u003c/em\u003esp. showed highest species density (0.5) among all the isolates. Isolation frequency was maximum (67%) in case of \u003cem\u003eFusarium \u003c/em\u003esp., \u003cem\u003eNigrospora \u003c/em\u003esp., \u003cem\u003ePenicillium chrysogenum \u003c/em\u003eand \u003cem\u003eRhizopus \u003c/em\u003esp. More fungal isolates were obtained from root and stem (47% each) as compared to leaves (29%). Species richness and diversity indices was maximum (15.0) in stem and minimum (9.0) in leaves. Highest Shannon and Simpson diversity index was in stem (2.02 and 0.860 respectively) followed by root (1.979 and 0.847 respectively) and least in leaves (1.494 and 0.75 respectively). Among the isolates tested for plant growth promoting parameters, EF09 (\u003cem\u003eFusarium\u003c/em\u003e sp.) showed positive response for all the tested parameters. The isolate, EF09 also showed maximum antifungal potential (up to 87.1%) against \u003cem\u003eP. theae\u003c/em\u003e in poisoned food technique. Enumeration of endophytic fungal diversity of \u003cem\u003eE. latifolia \u003c/em\u003eL.\u003cem\u003e \u003c/em\u003eholds promises as the putative strains may lead to the isolation of novel bioactive components for use in industry, medicine and agriculture.\u003c/p\u003e","manuscriptTitle":"Fungal Endophytes Associated With the Actinorhizal Plant Species, Elaeagnus Latifolia L. (Elaeagnaceae) And Evaluating Their Antagonistic Potential Against Grey Blight Disease In Tea [Camellia Sinensis (L.) O. 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