Biohydrogen production potential from the organic fraction of municipal solid waste in co- digestion with sewage sludge through high-solids dark fermentation

Biohydrogen production potential from the organic fraction of municipal solid waste in co- digestion with sewage sludge through high-solids dark fermentation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Biohydrogen production potential from the organic fraction of municipal solid waste in co- digestion with sewage sludge through high-solids dark fermentation Rodolfo Daniel Silva-Martínez, Oscar Aguilar-Juárez, Lourdes Díaz-Jiménez, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4638174/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Biohydrogen production from the organic fraction of municipal solid waste (OFMSW) in co-digestion with sewage sludge (SS) through dark fermentation (DF) is emerging as an alternative route for efficient waste treatment and biofuel production. Notwithstanding, there is little experience with a high-solid approach, where practical conditions are created to further develop feasible industrial-scale projects. This study conducted a biochemical hydrogen potential test using a 7L batch reactor at 35°C for 39 days. Parameters such as pH, redox potential, temperature, alkalinity, total solids, and substrate/inoculum ratio were adjusted and monitored. Biogas composition was analyzed by gas chromatography. The microbial characterization of SS and post-reaction percolate liquids was determined through metagenomics analyses. Results Results demonstrate an initial biohydrogen yield of 38.4 Nml/gVS OFMSW, which forms ~ 60% of the produced biogas. Aeration was proven as an efficient inoculum pretreatment method, mainly to decrease the levels of methanogenic archaea and metabolic competition, and at the same time maintain the required total solid (TS) contents for high-solid conditions. The microbial community analysis shows that the biohydrogen production was carried out by specific anaerobic and aerobic bacteria and dominated by the phylum Firmicutes such as the genus Bacillus, Clostridium, Romboutsia; and the phylum Proteobacteria with the genus Proteus. Conclusion These results aid in setting the base ground to further develop the technology and design a larger bioreactor for DF of OFMSW in co-digestion with SS, considering diverse parameters, the manufacturing process, and instrumentation. Thus, this research provides an alternative solution for OFMSW treatment and biohydrogen production and expects to serve as a basis for future research, which may intend to improve biochemical conditions to increase the amount of hydrogen produced, optimize biodegradation and, at the same time, develop an industrial high-solid dark fermentation system that is technically and economically viable. biohydrogen organic fraction municipal waste sewage sludge high solids dark fermentation Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4638174","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":328180489,"identity":"5bdcac56-3e6e-4cb8-b08d-435e3975135e","order_by":0,"name":"Rodolfo Daniel Silva-Martínez","email":"","orcid":"","institution":"Center for Research and Advanced Studies of the National Polytechnic Institute","correspondingAuthor":false,"prefix":"","firstName":"Rodolfo","middleName":"Daniel","lastName":"Silva-Martínez","suffix":""},{"id":328180490,"identity":"567b4d23-bd22-42e9-9176-9d031c42c740","order_by":1,"name":"Oscar 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fermentation","lastPublishedDoi":"10.21203/rs.3.rs-4638174/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4638174/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eBiohydrogen production from the organic fraction of municipal solid waste (OFMSW) in co-digestion with sewage sludge (SS) through dark fermentation (DF) is emerging as an alternative route for efficient waste treatment and biofuel production. Notwithstanding, there is little experience with a high-solid approach, where practical conditions are created to further develop feasible industrial-scale projects. This study conducted a biochemical hydrogen potential test using a 7L batch reactor at 35\u0026deg;C for 39 days. Parameters such as pH, redox potential, temperature, alkalinity, total solids, and substrate/inoculum ratio were adjusted and monitored. Biogas composition was analyzed by gas chromatography. The microbial characterization of SS and post-reaction percolate liquids was determined through metagenomics analyses.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eResults demonstrate an initial biohydrogen yield of 38.4 Nml/gVS OFMSW, which forms\u0026thinsp;~\u0026thinsp;60% of the produced biogas. Aeration was proven as an efficient inoculum pretreatment method, mainly to decrease the levels of methanogenic archaea and metabolic competition, and at the same time maintain the required total solid (TS) contents for high-solid conditions. The microbial community analysis shows that the biohydrogen production was carried out by specific anaerobic and aerobic bacteria and dominated by the phylum Firmicutes such as the genus Bacillus, Clostridium, Romboutsia; and the phylum Proteobacteria with the genus Proteus.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e \u003cp\u003eThese results aid in setting the base ground to further develop the technology and design a larger bioreactor for DF of OFMSW in co-digestion with SS, considering diverse parameters, the manufacturing process, and instrumentation. 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