Expression of CD117 and CD34 in uterine adenomyosis lesions

Liqun Guo; Wenjie Long; Lei Bao; Fudi Lang; Lingjia Huang; Hui Li; Ye Jin; Tian Hai; Mingli Huang

doi:10.3877/cma.j.issn.1673-5250.2017.03.015

Expression of CD117 and CD34 in uterine adenomyosis lesions

Liqun Guo, Wenjie Long, Lei Bao, Fudi Lang, Lingjia Huang, Hui Li, Ye Jin, Tian Hai, Mingli Huang

2017 · vol. 13(03) , pp. 326–331 · doi:10.3877/cma.j.issn.1673-5250.2017.03.015 · W3029440397

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This study investigated CD117 and CD34 expression in uterine adenomyosis, finding higher CD117 cell levels in lesions and suggesting CD117+ cells may contribute to adenomyosis pathogenesis.

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This study examined the expression and distribution of CD117 and CD34 proteins in uterine tissue to investigate their potential role in disease pathogenesis. Researchers compared immunohistochemical and immunofluorescence staining results from adenomyosis lesion tissues obtained from 54 patients against normal myometrial tissues from 50 controls. The findings revealed that CD117-positive cells were significantly more abundant in adenomyosis lesions than in normal tissue, where they localized around CD34-positive microvessels rather than within endothelial cells themselves. This paper is centrally about adenomyosis — specifically analyzing the expression of CD117+ cells and their spatial relationship with angiogenesis in adenomyotic lesions.

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Abstract

Objective To investigate the relationship between the distribution and quantity of CD117 in the disease tissue of adenomyosis and angiogenesis, to explore the relationship between CD117 cells and adenomyosis. Methods From July 2014 to December 2016 in the First Affiliated Hospital of Harbin Medical University, gynecological patients underwent hysterectomy for uterine myopathy in 54 patients with adenomyosis were selected as the study object and included in the uterine gland disease group. Select the same period in the hospital due to uterine fibroids or ovarian benign tumor hysterectomy in 50 patients with normal uterine muscle tissue into the control group (n=50). The expression of CD117 in adenomyosis and normal myometrial tissue was studied by immunohistochemical staining. The relationship between CD117 cells and uterus was studied by CD117 and CD34+ immunofluorescence staining. The procedures followed in this study are in line with the ethical standards set by the Human Body Test Committee of the First Affiliated Hospital of Harbin Medical University and are approved by the committee. The study obtained the informed consent of the subjects, and signed a clinical research informed consent. Results CD117 cells were found in the normal tissues of the adenomyosis group and the control group. CD117 cells expressed higher in the lesion tissues of the adenomyosis group than that in the control group. The average CD117 cells was (18±4), the control group was (8±3), the difference was statistically significant (t= 2.571, P<0.05). ② CD34+ and CD117 immunofluorescence staining showed that CD117 positive cells were CD117/CD34-cells under fluorescence microscope. There were more CD34+ microvascular structures in the tissues of adenomyosis, CD117 cells were distributed in the small blood vessels of adenomyosis, and CD34+ expression was found in the endothelial cells of the vessel wall, and no positive CD117+ expression was not found. Conclusion CD117+ cells may be involved in the pathogenesis of adenomyosis. Key words: Proto-oncogene proteins c-kit; Antigens, CD34; Adenomyosis; Aangiogenesis; Female

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Objective

To investigate the relationship between the distribution and quantity of CD117 in the disease tissue of adenomyosis and angiogenesis, to explore the relationship between CD117 cells and adenomyosis.

Methods

From July 2014 to December 2016 in the First Affiliated Hospital of Harbin Medical University, gynecological patients underwent hysterectomy for uterine myopathy in 54 patients with adenomyosis were selected as the study object and included in the uterine gland disease group. Select the same period in the hospital due to uterine fibroids or ovarian benign tumor hysterectomy in 50 patients with normal uterine muscle tissue into the control group (n=50). The expression of CD117 in adenomyosis and normal myometrial tissue was studied by immunohistochemical staining. The relationship between CD117 cells and uterus was studied by CD117 and CD34+ immunofluorescence staining. The procedures followed in this study are in line with the ethical standards set by the Human Body Test Committee of the First Affiliated Hospital of Harbin Medical University and are approved by the committee. The study obtained the informed consent of the subjects, and signed a clinical research informed consent.

Results

CD117 cells were found in the normal tissues of the adenomyosis group and the control group. CD117 cells expressed higher in the lesion tissues of the adenomyosis group than that in the control group. The average CD117 cells was (18±4), the control group was (8±3), the difference was statistically significant (t= 2.571, P<0.05). ② CD34+ and CD117 immunofluorescence staining showed that CD117 positive cells were CD117/CD34-cells under fluorescence microscope. There were more CD34+ microvascular structures in the tissues of adenomyosis, CD117 cells were distributed in the small blood vessels of adenomyosis, and CD34+ expression was found in the endothelial cells of the vessel wall, and no positive CD117+ expression was not found.

Conclusion

CD117+ cells may be involved in the pathogenesis of adenomyosis. | [1] | Wang PH, Su WH, Sheu BC, et al. Adenomyosis and its variance: adenomyoma and female fertility[J]. Taiwan J Obstet Gynecol, 2009, 48(3): 232-238. | | [2] | 蔡仕彬，何佳．子宫腺肌病发病机制及治疗研究进展[J]．川北医学院学报，2014，29(3): 305-309. | | [3] | 孙丽红. C-kit和TrkB在子宫腺肌病中的表达及其意义[D]. 济南：山东大学，2010. | | [4] | Verweij J, Judson I, van Oosterom A. STI571: a magic bullet？[J] Eur J Cancer, 2001, 37(15): 1816-1819. | | [5] | 姜亚卓，田普训，丁小明，等．小鼠骨髓CD117造血干细胞定向分化为未成熟树突状细胞及其鉴定[J]．南方医科大学学报，2007，27(4)：450-453. | | [6] | Ashman LK, Cambareri AC, To LB, et al. Expression of the YB5.B8 antigen (c-kit proto-oncogene product) in normal human bone marrow[J]. Blood, 1991, 78(1): 30-37. | | [7] | Nishida H, Daa T, Kashima K, et al. KIT (CD117) expression in benign and malignant sweat gland tumors[J]. Am J Dermatopathol, 2015, 37(12): 898-905. | | [8] | Ye JX, Liu Y, Qin Y, et al. Mast cells or not? CD117 positive cells in esophageal leiomyoma[J]. Histol Histopathol, 2015, 30(5): 581-588. | | [9] | John AM, Thomas NS, Mufti GJ, et al. Targeted therapies in myeloid leukemia[J]. Semin Cancer Biol, 2004, 14(1): 41-62. | | [10] | Sattler M, Salgia R. Targeting c-Kit mutations: basic science to novel therapies[J]. Leuk Res, 2004, 28(Suppl 1): S11-S20. | | [11] | Kerr BA, Miocinovic R, Smith AK, et al. CD117＋cells in the circulation are predictive of advanced prostate cancer[J]. Oncotarget, 2015, 6(3): 1889-1897. | | [12] | 谢幸，苟文丽．妇产科学.8版[M]．北京：人民卫生出版社，2013: 268-274. | | [13] | Uzan C, Cortez A, Dufournet C, et al. Endometrium from women with and without endometriosis, and peritoneal, ovarian and bowel endometriosis, show different c-kit protein expression[J]. J Reprod Immunol, 2005, 65(1): 55-63. | | [14] | Lam V, Kalesnikoff J, Lee CW, et al. IgE alone stimulates mast cell adhesion to fibronectin via pathways similar to those used by IgE＋ antigen but distinct from those used by steel factor[J]. Blood, 2003, 102(4): 1405-1413. | | [15] | Vosseller K, Stella G, Yee NS, et al. c-kit receptor signaling through its phosphatidylinositide-3′-kinase-binding site and protein kinase C: role in mast cell enhancement of degranulation, adhesion, and membrane ruffling[J]. Mol Biol Cell, 1997, 8(5): 909-922. | | [16] | Kalu E, Sumar N, Giannopoulos T, et al. Cytokine profiles in serum and peritoneal fluid from infertile women with and without endometriosis[J]. J Obstet Gynaecol Res, 2007, 33(4): 490-495. | | [17] | 汪玲莉. 子宫腺肌病发病的相关因素研究进展[J]．现代诊断与治疗，2014，25(5)：1177-1178. | | [18] | Mehasseb MK, Bell SC, Habiba MA. Neonatal administration of tamoxifen causes disruption of myometrial development but not adenomyosis in the C57/BL6J mouse[J]. Reproduction, 2010, 139(6): 1067-1075. | | [19] | Ota H. Evaluation of hysteroscopy in the diagnosis of adenomyosis[J]. Jpn J Fertil Steril, 1992, 37(1): 49-55. | | [20] | 陈世荣，胡志华，冯智君. SCF/C-Kit在子宫腺肌瘤和子宫内膜异位症患者在位和异位内膜中的表达和意义[J/CD]. 中华临床医师杂志(电子版), 2015(8): 45-48. | | [21] | Taylor RN, Yu J, Torres PB, et al. Mechanistic and therapeutic implications of angiogenesis in endometriosis[J]. Reprod Sci, 2009, 16(2): 140-146. | | [22] | May K, Becker CM. Endometriosis and angiogenesis[J]. Minerva Ginecol, 2008, 60(3): 245-254. | | [23] | 马美红，王辉. 子宫内膜异位症患者血清和腹腔液中肿瘤坏死因子及血管内皮生长因子水平的变化及意义[J]. 实用妇产科杂志，2009, 25(4): 223-226. | | [24] | 伍艳芳，何凌. 子宫内膜异位症患者血清VEGF和CRP水平变化及其相关性[J]. 现代生物医学进展，2009, 9(14): 2710-2712. | | [25] | Goscinski MA, Larsen SG, Giercksky KE, et al. PDGFR-α and CD117 expression pattern in esophageal carcinomas[J]. Anticancer Res, 2015, 35(7): 3793-3799. | | [26] | Safavi F, Li H, Gonnella P, et al. c-kit plays a critical role in induction of intravenous tolerance in experimental autoimmune encephalomyelitis[J]. Immunol Res, 2015, 61(3): 294-302. | | [27] | Takeyama A, Yoshikawa Y, Ikeo T, et al. Expression patterns of CD66a and CD117 in the mouse submandibular gland[J]. Acta Histochem, 2015, 117(1): 76-82. | | [28] | Salehinejad J, Mohtasham N, Bagherpour A, et al. Evaluation of c-kit protein (CD117) expression in common salivary gland neoplasms[J]. J Oral Maxillofac Pathol, 2014, 18(2): 177-182. | | [29] | Valentijn AJ, Palial K, Al-Lamee H, et al. SSEA-1 isolates human endometrial basal glandular epithelial cells: phenotypic and functional characterization and implications in the pathogenesis of endometriosis[J]. Hum Reprod, 2013, 28(10): 2695-2708. | | [30] | Horn LC, Meinel A, Hentschel B. c-kit/CD 117 positive cells in the myometrium of pregnant women and those with uterine endometriosis[J]. Arch Gynecol Obstet, 2012, 286(1): 105-107. | | [1] | 汪凯林, 李舜颖, 刘强. 第5版《年轻女性乳腺癌国际共识指南》更新要点解读[J/OL]. 中华乳腺病杂志(电子版), 2023, 17(02): 65-71. | | [2] | 张少华, 林爱齐, 张玉, 董晓云, 刘红英. 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