OsPPR8-1 is essential for early chloroplast development at 30 ℃

OsPPR8-1 is essential for early chloroplast development at 30 ℃ | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article OsPPR8-1 is essential for early chloroplast development at 30 ℃ Luo Chen, Xuezhong Li, Mingzhen Han, Qingjian Liu, Guowei Xie, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4295917/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Pentatricopeptide repeat (PPR) proteins regulate the expression of organelle genes in plants by participating in RNA metabolism. In this study, we identified a PPR gene OsPPR8-1 which encodes a typical P subfamily PPR protein with 9 PPR motifs. Compared to dicots, the OsPPR8-1 has a more closer relationship with monocots, such as brachypodium, sorghum, maize, and so on. Expression specificity analysis shows that OsPPR8-1 is highly expressed in green tissues and OsPPR8-1 was localized in chloroplasts. Phenotypic observation revealed that when the OsPPR8-1 gene undergone heterozygous mutations or lacking the one hundred and third (103rd) and one hundred and fourth (104th) amino acids, no significant phenotypic changes were observed. However, when the homozygous deletion of 1 bp, 2 bps or 38 bps in the CDS region of OsPPR8-1 which caused frameshift mutations, the rice seedlings show an albino phenotype from germination to the third-leaf stage, and then gradually died at 30 ℃. All chlorophyll a (Chla), chlorophyll b (Chlb), carotenoid (Car) and total chlorophyll (Chl) significantly decreased in osppr8-1 homozygous mutants, and ultrathin section shows that OsPPR8-1 participates in regulating the generation of chloroplasts. Loss-of-function of OsPPR8-1 resulted in severe defects in the intron splicing of PetB, atpF, trnG and rps12. These results indicate that OsPPR8-1 is indispensable for early chloroplast development and seedling growth in rice by influencing the intron splicing of multiple chloroplast group II introns at 30 ℃. OsPPR8-1 PPR protein chloroplast development Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4295917","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":298347134,"identity":"33b7ce6a-ee23-4b16-93b4-78b4e94c0331","order_by":0,"name":"Luo Chen","email":"","orcid":"","institution":"Zhongkai University of Agriculture and Engineering","correspondingAuthor":false,"prefix":"","firstName":"Luo","middleName":"","lastName":"Chen","suffix":""},{"id":298347135,"identity":"8590c71e-6050-4dbc-a299-05457939250f","order_by":1,"name":"Xuezhong Li","email":"","orcid":"","institution":"Zhongkai University of Agriculture and 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development","lastPublishedDoi":"10.21203/rs.3.rs-4295917/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4295917/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Pentatricopeptide repeat (PPR) proteins regulate the expression of organelle genes in plants by participating in RNA metabolism. In this study, we identified a PPR gene OsPPR8-1 which encodes a typical P subfamily PPR protein with 9 PPR motifs. Compared to dicots, the OsPPR8-1 has a more closer relationship with monocots, such as brachypodium, sorghum, maize, and so on. Expression specificity analysis shows that OsPPR8-1 is highly expressed in green tissues and OsPPR8-1 was localized in chloroplasts. Phenotypic observation revealed that when the OsPPR8-1 gene undergone heterozygous mutations or lacking the one hundred and third (103rd) and one hundred and fourth (104th) amino acids, no significant phenotypic changes were observed. However, when the homozygous deletion of 1 bp, 2 bps or 38 bps in the CDS region of OsPPR8-1 which caused frameshift mutations, the rice seedlings show an albino phenotype from germination to the third-leaf stage, and then gradually died at 30 ℃. All chlorophyll a (Chla), chlorophyll b (Chlb), carotenoid (Car) and total chlorophyll (Chl) significantly decreased in osppr8-1 homozygous mutants, and ultrathin section shows that OsPPR8-1 participates in regulating the generation of chloroplasts. Loss-of-function of OsPPR8-1 resulted in severe defects in the intron splicing of PetB, atpF, trnG and rps12. 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