Topological Regulation of the Mammalian Genome by Positive DNA Supercoiling

Abstract DNA supercoiling is a fundamental aspect of genome topology characterizing all DNA transactions. While negative supercoiling has been extensively characterized, positive supercoiling remains poorly understood. Using a quantitative GapR profiling system, we establish comprehensive maps of positive supercoiling across interphase and mitosis. We show that positive super-coils accumulate not only at gene ends but prominently at promoters, enhancers, insulators and loop anchors, where they are resolved by Topoisomerases. Biochemical and functional assays reveal the main sources of positive torsion: transcription generates genic supercoils, while R-loops and Cohesin drive accumulation at regulatory elements, topologically associating domains and their boundaries. During mitotic chromosomal compaction, Condensins establish a global wave of positive supercoiling that largely homogenizes the genome, yet promoters with rapid post-mitotic reactivation retain elevated torsion and R-loops. These findings establish positive DNA supercoiling as a form of topological memory that links DNA mechanics to transcriptional control, genome architecture and epigenetic inheritance. Competing Interest Statement The authors have declared no competing interest.