A Novel Cell-Based Multiplex Immunoassay Platform
preprint
OA: closed
CC-BY-4.0
Abstract
Abstract We describe generation of stable, fluorescence-barcoded cell lines suitable for multiplex screening of antibody to membrane proteins. The utility of this cell-based system, capable of a 256-plex cell panel, is demonstrated by flow cytometry deconvolution of barcoded cell panels expressing influenza A hemagglutinin trimers, or native human CCR2 or CCR5 multi-spanning proteins and their epitope-defining mutants. This platform may prove useful for characterizing immunity and discovering antibodies to membrane-associated proteins.
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Source provenance
- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00
- unpaywall
- last seen: 2026-05-30T02:00:01.510937+00:00
License: CC-BY-4.0