MicroRNA expression analysis in endometriotic serum treated mesenchymal stem cells

Abdel-Rasheed, Mazen, Nour Eldeen, Ghada, Mahmoud, Marwa, ElHefnawi, Mahmoud, Abu-Shahba, Nourhan, Reda, Mohamed, Elsetohy, Khaled, Nabil, Michael, Elnoury, Amr, Taha, Tamer, Azmy, Osama
2017 · doi:10.17877/de290r-18200 · W6906732942
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Treating human endometrial mesenchymal stem cells with serum from severe endometriosis patients dysregulated 32 microRNAs, potentially altering cell functions and contributing to endometriosis development.

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This study investigated the impact of serum from women with severe endometriosis on microRNA expression in human endometrial mesenchymal stem cells. Researchers cultured these cells with patient-derived sera and used PCR arrays to analyze 84 specific microRNAs, comparing them against controls using serum from healthy women. The analysis identified thirty-two significantly dysregulated microRNAs whose target genes were enriched in biological processes associated with endometriosis pathophysiology. These findings indicate that exposure to endometriotic serum alters miRNA profiles in stem cells, potentially driving the abnormal differentiation linked to disease development. This paper is centrally about endometriosis — specifically examining how serum factors influence mesenchymal stem cell behavior relevant to lesion formation.

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Abstract

Endometriosis is defined by presence of endometrial-like-tissue outside the uterus. Recently, ectopic endometriotic lesions have been suggested to originate by abnormal differentiation of endometrial mesenchymal stem cells (eMSCs). MicroRNAs (miRNAs) play an important role in the pathophysiology of endometriosis. Through a PCR array approach, we aimed to assess the differential expression of microRNAs in human eMSC treated in culture with sera derived from women with severe endometriosis. Sera were collected from five patients with severe endometriosis and three control women and added individually in the culture medium to conduct experimental and control eMSC sets, respectively. Regular microscopic follow-up for cell morphology was performed. SYBR Green based real-time PCR array was used to assess the expression of 84 miRNAs. Bioinformatics analysis was done to predict the target genes of the significantly dysregulated miRNAs and their enriched biological processes and pathways. Thirty-two miRNAs were found significantly dysregulated in experimental cultures. Functional enrichment analysis revealed several endometriosis associated biological processes and pathways were enriched by target genes of these miRNAs. In conclusion, treatment of human eMSCs with sera of severe endometriosis cases affects the expression of certain miRNAs and their target genes. This may result in altering cell functions and consequently, endometriosis development.
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Abstract

Endometriosis is defined by presence of endometrial-like-tissue outside the uterus. Recently, ectopic endometriotic lesions have been suggested to originate by abnormal differentiation of endometrial mesenchymal stem cells (eMSCs). MicroRNAs (miRNAs) play an important role in the pathophysiology of endometriosis. Through a PCR array approach, we aimed to assess the differential expression of microRNAs in human eMSC treated in culture with sera derived from women with severe endometriosis. Sera were collected from five patients with severe endometriosis and three control women and added individually in the culture medium to conduct experimental and control eMSC sets, respectively. Regular microscopic follow-up for cell morphology was performed. SYBR Green based real-time PCR array was used to assess the expression of 84 miRNAs. Bioinformatics analysis was done to predict the target genes of the significantly dysregulated miRNAs and their enriched biological processes and pathways. Thirty-two miRNAs were found significantly dysregulated in experimental cultures. Functional enrichment analysis revealed several endometriosis associated biological processes and pathways were enriched by target genes of these miRNAs. In conclusion, treatment of human eMSCs with sera of severe endometriosis cases affects the expression of certain miRNAs and their target genes. This may result in altering cell functions and consequently, endometriosis development. Description Table of contents

Keywords

endometriosis, mesenchymal stem cells, miRNA expression, differentiation

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endometriosis

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