The pentraxin 3 level profile of ovarian follicle fluid in infertile patients with endometriosis

Endometriosis gives the most proportion as the cause of infertility. One of the factor in endometriosis suspected to be the cause of infertility is the decrease of oocyte quality, which is determined by cumulus expansion. Cumulus cell expansion indirectly causes the production of hyaluronic acid (HA), prostaglandin E2 (PGE2), pentraxin (PTX3), and tumor necrosis factor alpha-induced protein 6 (TNF-aIP6). The objective of this study was to prove the difference in Pentraxin3 level of ovarian follicle fluid in infertile patients with severe stage endometriosis, mild stage endometriosis and without endometriosis. This study was held in Fertility Clinic of Graha Amerta dr. Sutomo Hospital, Surabaya, and Biochemistry Laboratory of Science Faculty Brawijaya University, Malang. This study was an analytic-observational study in human with cross-section study design on follicle fluid samples by observing and measuring the variable from study subject follicle fluid of infertile women that is diagnosed using laparoscopy. The measurement of Pentraxin-3 level on ovarian follicle fluid was done by ELISA. We obtained 300 samples based on laparoscopic findings, The sample were categorized into three groups: Non-endometriosis infertile/control, mild endometriosis infertile and severe endometriosis infertile. The total mean PTX level was -3 3.96 ± 2.71 ng/ml. With the level of PTX-3 on control group 1.21 ± 0.23 ng/ml, mild endometriosis 3.47 ± 0.38 ng/ml, and severe endometriosis 7.21 ± 1.73 ng/ml. Correlation analysis between Pentraxin-3 level with endometriosis severity rs = 0.943 and p = 0.001, showed high correlation. In conclusion, the more severe endometriosis is the level of Pentraxin-3 is also higher. (MOG 2012;20:8-13)