LILAC: Enhanced actin imaging with an optogenetic Lifeact
preprint
OA: closed
CC-BY-NC-ND-4.0
Abstract
We have designed an improved Lifeact variant that binds to actin under the control of light using the LOV2 protein. Light control enables one to subtract the pre-illumination signal of the unbound label, yielding an enhanced view of F-actin dynamics in cells. Furthermore, the tool eliminates actin network perturbations and cell sickness caused by Lifeact overexpression.
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- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00
- unpaywall
- last seen: 2026-05-30T02:00:01.510937+00:00
License: CC-BY-NC-ND-4.0