Evaluation of the plasma oxylipin and endocannabinoid profile and the platelet and plasma proteome in postpartum dairy cows experiencing elevated systemic inflammation

Evaluation of the plasma oxylipin and endocannabinoid profile and the platelet and plasma proteome in postpartum dairy cows experiencing elevated systemic inflammation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Evaluation of the plasma oxylipin and endocannabinoid profile and the platelet and plasma proteome in postpartum dairy cows experiencing elevated systemic inflammation Jillian M. Grantz, Venkatesh P. Thirumalaikumar, Amber H. Jannasch, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4420521/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 28 Dec, 2024 Read the published version in Scientific Reports → Version 1 posted 9 You are reading this latest preprint version Abstract Unregulated, systemic inflammation negatively impacts health and production in dairy cows. Soluble mediators and platelets have been studied for their expansive role in mediating inflammation. Our objectives were to compare the plasma oxylipin and endocannabinoid profiles, and the platelet and plasma proteomic profiles of healthy cows to cows suffering from elevated systemic inflammation as indicated by plasma haptoglobin (Hp) concentrations. Postpartum cows at 3 DIM with plasma Hp concentrations \(\ge\) 0.50 g/L and no clinical disease were enrolled into the high-inflammation group (n = 8). Cows with plasma Hp concentrations \(\le\) 0.1 g/L and no clinical disease were enrolled into the low-inflammation group (n = 8). Targeted lipidomic analysis revealed differences in the plasma oxylipin and endocannabinoid profile between high- and low-inflammation cows. Cows in the high-inflammation group had increased concentrations of the oxylipins 9(S)-HpOTrE, 9(S)-HOTrE, 13(S)-HpOTrE, and 9,10-EpOME, and the endocannabinoid anandamide, in plasma. In-depth proteomic analysis of platelets between the high- and low-inflammation groups revealed significant differences in protein categories related to platelet granule release and cellular iron uptake. Proteomic outputs from plasma revealed 24 proteins to be different between high and low-inflamed groups, including proteins involved in autophagy and immune mediation. Together, our results indicate that cows suffering from an exacerbated systemic inflammatory response in the postpartum may have impaired disease resistance, and platelets could contributors to their inflammatory state. Biological sciences/Immunology Health sciences/Diseases/Immunological disorders/Inflammatory diseases Systemic inflammation Haptoglobin Platelet Oxylipin Endocannabinoid Full Text Additional Declarations No competing interests reported. Supplementary Files Grantzetal.PlateletStudyManuscriptSupplementalMaterial05.14.2024SubmittedVersion.docx SupplementaryFile1PlateletandPlasmaProteomics05.14.2024SubmittedVersion.xlsx SupplementaryFile2PlateletandPlasmaProteomics05.14.2024SubmittedVersion.xlsx Cite Share Download PDF Status: Published Journal Publication published 28 Dec, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 22 Jul, 2024 Reviews received at journal 03 Jun, 2024 Reviewers agreed at journal 29 May, 2024 Reviewers agreed at journal 29 May, 2024 Reviewers invited by journal 29 May, 2024 Editor assigned by journal 21 May, 2024 Editor invited by journal 21 May, 2024 Submission checks completed at journal 21 May, 2024 First submitted to journal 14 May, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4420521","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":308310367,"identity":"588e8879-00cd-4e4b-84b8-06200f836658","order_by":0,"name":"Jillian M. 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Soluble mediators and platelets have been studied for their expansive role in mediating inflammation. Our objectives were to compare the plasma oxylipin and endocannabinoid profiles, and the platelet and plasma proteomic profiles of healthy cows to cows suffering from elevated systemic inflammation as indicated by plasma haptoglobin (Hp) concentrations. Postpartum cows at 3 DIM with plasma Hp concentrations \u003cspan class=\"InlineEquation\"\u003e\u003cspan class=\"mathinline\"\u003e\\(\\ge\\)\u003c/span\u003e\u003c/span\u003e 0.50 g/L and no clinical disease were enrolled into the high-inflammation group (n\u0026thinsp;=\u0026thinsp;8). Cows with plasma Hp concentrations \u003cspan class=\"InlineEquation\"\u003e\u003cspan class=\"mathinline\"\u003e\\(\\le\\)\u003c/span\u003e\u003c/span\u003e 0.1 g/L and no clinical disease were enrolled into the low-inflammation group (n\u0026thinsp;=\u0026thinsp;8). Targeted lipidomic analysis revealed differences in the plasma oxylipin and endocannabinoid profile between high- and low-inflammation cows. Cows in the high-inflammation group had increased concentrations of the oxylipins 9(S)-HpOTrE, 9(S)-HOTrE, 13(S)-HpOTrE, and 9,10-EpOME, and the endocannabinoid anandamide, in plasma. In-depth proteomic analysis of platelets between the high- and low-inflammation groups revealed significant differences in protein categories related to platelet granule release and cellular iron uptake. Proteomic outputs from plasma revealed 24 proteins to be different between high and low-inflamed groups, including proteins involved in autophagy and immune mediation. Together, our results indicate that cows suffering from an exacerbated systemic inflammatory response in the postpartum may have impaired disease resistance, and platelets could contributors to their inflammatory state.\u003c/p\u003e","manuscriptTitle":"Evaluation of the plasma oxylipin and endocannabinoid profile and the platelet and plasma proteome in postpartum dairy cows experiencing elevated systemic inflammation","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-06-03 18:52:25","doi":"10.21203/rs.3.rs-4420521/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-07-23T03:59:57+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-06-03T20:33:14+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"158701570453890482844220552323881514959","date":"2024-05-29T16:07:43+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"286210668991068325577582877499340266107","date":"2024-05-29T15:48:28+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-05-29T15:27:57+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-05-21T13:28:27+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2024-05-21T12:16:15+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-05-21T12:14:27+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2024-05-14T16:08:05+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"f9ef7575-8850-4564-aa71-ef0ecd8ae780","owner":[],"postedDate":"June 3rd, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":32569541,"name":"Biological sciences/Immunology"},{"id":32569542,"name":"Health sciences/Diseases/Immunological disorders/Inflammatory diseases"}],"tags":[],"updatedAt":"2024-12-30T16:01:41+00:00","versionOfRecord":{"articleIdentity":"rs-4420521","link":"https://doi.org/10.1038/s41598-024-82553-x","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2024-12-28 15:57:34","publishedOnDateReadable":"December 28th, 2024"},"versionCreatedAt":"2024-06-03 18:52:25","video":"","vorDoi":"10.1038/s41598-024-82553-x","vorDoiUrl":"https://doi.org/10.1038/s41598-024-82553-x","workflowStages":[]},"version":"v1","identity":"rs-4420521","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4420521","identity":"rs-4420521","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}