Phosphorus uptake and PSR gene expression in eucalypts plants under split root system

Phosphorus uptake and PSR gene expression in eucalypts plants under split root system | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Phosphorus uptake and PSR gene expression in eucalypts plants under split root system Luana FerreiraTorres, Sara Adrián López de Andrade, Paulo Mazzafera This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4365628/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 3 You are reading this latest preprint version Abstract Phosphorus (P) often limits plant growth and development because its availability in most soils is low, mainly in tropical soils. Various phosphate transporters and regulatory elements, including transcription factors, are involved in the uptake and transport of P from the soil into root cells and to other plant organs. The split-root technique was applied to three eucalypt species to understand better the mechanisms of the root-leaf signaling and remobilization response to P supply. Two-month-old seedlings of Eucalypts grandis, E. globulus, and E. tereticornis were used, with each half of the split root system placed in pots. The P treatments were: +P/+P, +P/-P, and-P/-P (+P = P supplementation and –P = P depleted). P was supplied as 440 μM nutrient solution. Eucalypts plants were grown for six weeks and RT-qPCR evaluated the expression of genes related to P uptake, transport, and utilization in roots and leaves. P supply on one side of the root seemed to compensate for the lack of P on the other side in the +P/-P treatment, so the plant did not show P stress responses, and root-to-root signaling and remobilization in this treatment differed depending on the species. The genes analyzed were mostly induced when plants were under P deprivation, and the expression response was species-dependent. Therefore, this indicates that different mechanisms may be involved in plant response to low P and that signaling control may also be linked to the adaptation of eucalypts species to low soil P. Phosphorus stress plant nutrition split-root PSR phosphate transporters. Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editor assigned by journal 04 May, 2024 Submission checks completed at journal 03 May, 2024 First submitted to journal 03 May, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4365628","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":299381220,"identity":"482a9543-9fd8-44f1-8304-e887568e3bf0","order_by":0,"name":"Luana FerreiraTorres","email":"","orcid":"","institution":"State University of Campinas","correspondingAuthor":false,"prefix":"","firstName":"Luana","middleName":"","lastName":"FerreiraTorres","suffix":""},{"id":299381222,"identity":"19cbf26a-8983-4865-8c70-70ca30347e48","order_by":1,"name":"Sara Adrián López de Andrade","email":"","orcid":"","institution":"State University of Campinas","correspondingAuthor":false,"prefix":"","firstName":"Sara","middleName":"Adrián López","lastName":"de Andrade","suffix":""},{"id":299381225,"identity":"2becd03a-2bb7-49de-9004-1ec3364ff97c","order_by":2,"name":"Paulo Mazzafera","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAwElEQVRIiWNgGAWjYPACGwYGZggrgVgtaaRrOQxnEdZiLnb48ccfNecTt7MzP/x0cwdDnnkDAS2Ws9MMDCSO3U7c2cxmLJ17hqFY5gABLQa3EwwSDNhuJ244zMMgndvGkDiDkMMMbqd/OJDw7xxIC/NvIrXkGDYcbDsA0sJGnC2Ws3OKGRv7ko03HGYzs849I1EsQUiLuXT65o8/vtnJbjh/+PHt3B02eQS1GKDwGBsIasDUQljHKBgFo2AUjDwAAO+AQXch3DjwAAAAAElFTkSuQmCC","orcid":"","institution":"State University of Campinas","correspondingAuthor":true,"prefix":"","firstName":"Paulo","middleName":"","lastName":"Mazzafera","suffix":""}],"badges":[],"createdAt":"2024-05-03 18:55:20","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4365628/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4365628/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":56161644,"identity":"e8654502-5741-4d71-828c-c753c25c0718","added_by":"auto","created_at":"2024-05-09 09:37:13","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":931132,"visible":true,"origin":"","legend":"","description":"","filename":"AgroforestryLuana.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4365628/v1_covered_edb58636-732d-414a-90dc-9fcfb06de8c4.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Phosphorus uptake and PSR gene expression in eucalypts plants under split root system","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"agroforestry-systems","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"agfo","sideBox":"Learn more about [Agroforestry Systems](http://link.springer.com/journal/10457)","snPcode":"10457","submissionUrl":"https://submission.nature.com/new-submission/10457/3","title":"Agroforestry Systems","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Phosphorus stress, plant nutrition, split-root, PSR, phosphate transporters.","lastPublishedDoi":"10.21203/rs.3.rs-4365628/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4365628/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Phosphorus (P) often limits plant growth and development because its availability in most soils is low, mainly in tropical soils. 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