MOESM1 of Mass cytometry analysis reveals a distinct immune environment in peritoneal fluid in endometriosis: a characterisation study

Additional file 1: Lists of antibody and patients information used in this study and additional figures in support of the mass cytometry analysis. Table S1. Related to Fig. 1 and Experimental Procedures. Details of antibodies used in this study. Table S2. Related to Fig. 1. Information of 20 patients from whom PF and blood are used in this study. Table S3. Related to Fig. 1, Fig. 2 and Fig. 6. Markers used to define immune cell types. Table S4. Related to Fig. 4 and Experimental Procedures. Antibodies used in the analysis of 38 PF samples. Table S5. Related to Fig. 4. Information of 38 patients in follow up study. Table S6. Related to Fig. 6 and Experimental Procedures. Antibodies used in the T cell panel. Table S7. Related to Fig. 6. Information of 11 patient samples used in T cell panel study. Figure S1. Graphic workflow of CyTOF study comparing PFCs and PBCs. Figure S2 and Figure S3. Related to Fig. 1, Fig. 2 and Fig. 6. Manual gating of cells subsets and functional markers. Figure S4. Related to Fig. 1. Clustering of PF and blood samples by PCA. Figure S5. Related to Fig. 1. Phenotypic mapping of PBCs. Figure S6. Related to Fig. 2. Percentage of major immune cells types in blood and PF samples and expression of functional markers. Figure S7. Related to Fig. 2. Cell counts show changes of major cell populations in PF compared to peripheral blood. Figure S8. Related to Fig. 3. Differential expression of CD69 in endometriosis was not affected by menstruation or hormone. Figure S9. Related to Fig. 4. Cell counts of major cell subtypes in PFCs at disease stages and evaluation of confounding effects from menstrual cycle and hormones. Figure S10. Related to Fig. 4. A. PCA separates endometriosis (Endo) and control in PF but not blood samples. Figure S11. Related to Fig. 6. ViSNE plot showing composition of T cells and comparison of CD69 abundance on T cell lineages between control and endometriosis samples from PF.