CORRELLATIONS BETWEEN EXPRESSION NLRP3- INFLAMMASOME AND GYNECOLOGICAL PATHOLOGY IN WOMEN WITH ENDOMETRIOSIS ASSOCIATED INFERTILITY

The aim of our study was to examine the expression NLRP3-infl ammasome and some indices in the blood of women with endometriosis- associated infertility using assisted reproductive technologies with included probiotic.Material and methods: For the purpose of the research we examined 30 infertile women. Control group consisted of 10 women with tubal infertility due to an infl ammatory process in the anamnesis, in whom, as a result of a complex clinical and laboratory examination, no other diseases were detected and in terms of their health they could be equated with practically healthy women (age from 21 to 42 years, the average age was 29.75 years) and who did not take probiotic. The main group consists from 20 women with external genital endometriosis were included in assisted reproductive technologies. Patients from main group took probiotic 1 tablet twice a day with 10Ч109 Lactobacillus during 1 month in complex preparation (treatment) before assisted reproductive technologies. The indices of NLRP3-infl ammasome determined before and after preparation. The study was performed in Bukovinian State Medical University and Centre of Reproductive Medicine. The primary infertility incidence was signifi cantly higher in patients from main group. To analyze the expression of the NLPP3-infl ammasome gene and determine the relative normalized expression of mRNA NLPP3-infl ammasome, we used the polymerase chain reaction with reverse transcription in real time (RT-PCR). The object for molecular genetic studies by the RT-PCR method was the fraction of mononuclear cells isolated from the whole blood of patients with endometriosis. The value of p (authenticity diff erence) was determined by Student’s table- Fischer. Diff erences between contrasting averages were considered signifi cant at p <0.05.Results and discussion: Analyzed the results of our research stated that in main group expression of NLRP3-infl ammasome was 24.43, which is signifi cantly higher than after preparation (0,70 accordingly). In control group expression of NLRP-3-infl ammasome was 0.54.Expression of NLRP3-infl ammasome increased in patients before preparation more than 34 times compare with patients after preparation accordingly.Conclusions. Consequently, after using probiotic, the increased level of NLRP3-infl ammasome sharply decreases, indicating the eff ectiveness and the possibility of use in the program for assisted reproductive technologies preparation.