Multi-omics integration analysis and association study reveal the potential of ADIPOQ function in gestational diabetes mellitus

Abstract AimTo investigate the role of ADIPOQ gene in gestational diabetes mellitus (GDM). Methods We genotyped SNPs rs266729 and rs1501299 of ADIPOQ gene in1157 pregnant women of north Chinese Han population. Additionally, we used conventional bioinformatics analysis methods to conduct multi-omics analysis (transcriptome, epigenome, and single cell level) of ADIPOQ regulated GDM. Results The fetal umbilical artery doppler systolic blood flow velocity/diastolic blood flow velocity (S/D) ratio of umbilical artery in GDM patients with CC genotype of rs266729 and GG genotype of rs1501299 was higher than control. Single cell analysis suggested that ADIPOQ was expressed in extravillous trophoblast (EVT), T cell, monocytes, myelocyte, NK cell and syncytiotrophoblast (SCT). Functional enrichment analysis showed ADIPOQ gene was associated with response to nutrient levels, fat cell differentiation. Conclusion The findings of our study indicate a correlation between SNPs of ADIPOQ in GDM patients, and ADIPOQ is involved in the transcriptional regulation of GDM.