Evaluation of Antimicrobial Activity of Coleus forskohlii on Periodontal Pathogenic Bacteria, An experimental in-vitro study

Evaluation of Antimicrobial Activity of Coleus forskohlii on Periodontal Pathogenic Bacteria, An experimental in-vitro study | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Evaluation of Antimicrobial Activity of Coleus forskohlii on Periodontal Pathogenic Bacteria, An experimental in-vitro study Amel Ibrahim Faragalla, Ghada Khalid Bahamdan, Aseelah Abdullah, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3796502/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background: Coleus Forskohlii (C.F.) is a typical indigenous medicinal plant from the Lamiaceae family grown widely in India. Coleus gained importance after discovering its therapeutic properties (Bone, 2007 ) and was reported to have some antibacterial activity. The current study tested the antibacterial effect of (C F) on certain periodontal pathogens. Methods An experimental Invitro-study was conducted at King Khalid University, to test the antibacterial activity of Coleus Forskohlii. Thirty plaque samples were collected from gingivitis patients, kept, and transported using nutrient broth to the microbiology lab. The (CF) extract was prepared from the plant's leaves and five solutions were produced (water, methanol, ethyl acetate, chloroform, and hexane). Following the bacterial culturing, the extract solutions were applied and the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) were measured. Results Various activities were demonstrated, indicating the positive inhibitory reactions of ethanolic extracts against many bacteria grown directly from plaque samples. Twenty-five bacterial species have been inhibited at 1g / mL concentrations. Most organisms (82.9%) have been inhibited at 1g / mL, whereas 34.3% were inhibited at 0.25 g/ mL, and only 5.7% were inhabited at 0.15 g/ mL; the latter two concentrations represented the MIC for most of the strains. Conclusion The ethanolic extract of Coleus forskohlii demonstrated effective antimicrobial activity. The ethanolic extract and water extracts showed different activities against the selected bacteria, but the ethanolic extract was superior to the water extract. Aerobic pathogens (Bone 2007): Coleus forskohlii Ethanol Gingivitis Plant extract Figures Figure 1 Figure 2 Figure 3 Introduction Medicinal plants are a rich source of antibacterial agents and curatives. Coleus Forskohlii (C.F) is an important medicinal plant, achievable, non-toxic, and non-irritant used by humans to manage systemic diseases like respiratory and cardiac disorders and skin infections (Bone, 2007). Coleus forskohlii (Lamiaceae), a traditional plant of South India, is a source of bio-reductants and stabilizers (Malleswari et al., 2013). Coleus forskohlii stimulates cyclic adenosine monophosphate, regulates the number of enzymes, and develops some antibacterial effects (Batista et al., 1995). It has biological activities (Caprioli et al., 1984) (Bauer et al., 1993). Some studies reported using C. F. to treat systemic cardiovascular, respiratory disorders, and congestive heart failure (Shivaprasad et al., 2014). It has antimicrobial, antioxidant, and many pharmacological activities (Shivaprasad et al., 2014). The essential oil derived from Coleus forskohlii showed antimicrobial activity against streptococcus species (Majeed and Prakash, 2003). Due to the presence of a wide range of periodontal pathogens and the development of bacterial resistance, the current study tested the antibacterial activity of Coleus Forskohlii against aerobic periodontal pathogens. It aimed to determine the Coleus forskohlii effect on the aerobic bacterial pathogen that initiates gingivitis by collecting plaque samples. However, to our knowledge, no previous study was conducted to test (CF) antibacterial activity against aerobic periodontal pathogens. Methods An experimental Invitro-study was conducted at the Periodontic Clinic, College of Dentistry, King Khalid University, Abha, Saudi Arabia. This study was approved by the University's Institutional Review Board and provided the registration number IRB/REG/2022-2023/3. The study was according to the ethical principles of the World Medical Association Declaration of Helsinki (2013). Volunteers signed the consent form before participation. Thirty plaque samples were collected from male and female gingivitis patients seeking periodontal treatment. A sterile paper point size (40) was inserted in the gingival sulcus for 15 seconds. Then, samples were kept and transported using nutrient broth media suitable for aerobes to the microbiology lab. Coleus forskohlii leaves were collected from the Aseer region, Abha Kingdom of Saudi Arabia, in October 2022. The leaves were carefully rinsed with distilled water, dried overnight in a hot air oven at 40°C, and ground to a particle size of 40 mesh by using mortar and pestle. The plant powder (32.6 g) was divided into five portions, and each portion was extracted with a solvent, including water, methanol, ethyl acetate, chloroform, and hexane by decoction for two hours, followed by maceration for 48 hours to get five extracts. Assay for the antimicrobial activity of Coleus forskohlii extract was done by the disk impregnation method with minor modification from the following standard methods (Breakpoints, 2011) : One to three loopful of 24 hold cultures from each test strain were used to prepare 0.5 McFarland standard suspensions. A loopful of 0.5 McFarland suspensions was used to streak Mueller Hinton’s agar plates (Difco). Discs were placed on the inoculated agar, and inoculated plates were incubated at 37°C and examined after 24, 48, and 72 hours to detect the inhibition zones. Inhibition zones were measured in mm by a ruler for each bacterial strain under and around discs. The minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) have been considered valuable endpoints of antibacterial activity to guide effective therapy of bacterial infections. The disc impregnated discs at various concentrations have been tested to determine the MIC. Results The antimicrobial activity of ethanolic and water extract of Coleus forskohlii against clinical and reference bacterial strains is shown in Table 1 and Figures 1, 2, and 3. Isolation and the preliminary naming standard were performed as per routine laboratory methods and following standard protocols (Guo-Xin et al., 2014). The identity of isolates was confirmed using the Vitek 2 automated system according to manufacturer procedures (bio Mérieux VITEK, Inc.). Vitek 2 was also used to test their antimicrobial sensitivities. The ethanolic and water extracts showed different activities against the selected bacteria, but the ethanolic extract was superior to the water extract. A wide spectrum inhibitory activity was shown against beta-hemolytic Streptococcus pyogenes, an isolate from plaque samples. The activity against Staphylococcus aureus and E. coli was less than that against Streptococcus pyogenes. The direct in-vitro assay was applied to 10 clinical samples grown on Tryptocase Soy broth. The grown cultures were tested directly against the ethanolic extract of Coleus forskohlii. Various activities were demonstrated, indicating the positive inhibitory reactions of Coleus forskohlii ethanolic extracts against many bacteria grown directly from plaque samples. Direct application of an ethanolic extract of Coleus forskohlii indicated a good inhibitory antagonism to many unidentified bacteria grown from plaque samples. Twenty-five bacteria have been inhibited at a concentration of 1g / mL. Most (82.9%) of the organisms have been inhibited at 1g / mL, whereas 34.3% were inhibited at 0.25 g/ mL, and only 5.7% were inhabited at 0.15 g/ mL; the latter two concentrations represented the MIC for most of the strains. Table 1. In vitro assay of Coleus forskohlii extract against representatives gram-positive and gram-negative (zone of inhibition in mm). Figure 1. Some bacterial strains are susceptible to E. coli (left) and Staphylococcus aureus (right) to 1:10 concentration of Coleus forskohlii ethanolic and water extracts. Note the narrow inhibition zone of the ethanolic extract. Figure 2. Showed Susceptibility of Streptococcus pyogenes to 1:10 concentration of Coleus forskohlii ethanolic and water extracts. There was a wide inhibition zone of the ethanolic extract compared to a relatively smaller zone in the case of the water extract. Figure 3. Showed direct susceptibilities of isolates from patient gingivitis to 1:10 concentration of Coleus forskohlii ethanolic and water extracts. Note the narrow inhibition zone of the ethanolic extract. Note the clearance (inhibitions zone) of some plates indicating the collective action of Coleus forskohlii extracts against unidentified isolates (direct method). Discussions In the current study, five extracts of Coleus forskohlii were tested for antimicrobial activity against beta-hemolytic Streptococcus pyogenes , a Gram-positive, aerotolerant bacteria in the genus Streptococcus . These bacteria are extracellular and comprise non-motile and non-sporing cocci (round cells) that tend to link in chains. They are clinically important for humans, as they are infrequent but usually pathogenic (Clemons and Queen). E. coli and Staphylococcus aureus all were isolated from plaque samples. Assessed by the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC). Ethanolic and water extracts showed a significant wide-spectrum inhibitory activity and reduction of the tested organism. In agreement with this study, Pullaiah tested the activity of Coleus forskohlii in an oil extract against Staphylococcus aureus, E. coli Staphylococcus aureus, streptococcus species , and other aerobic bacterial pathogens and concluded with the presence of antibacterial activity of Coleus forskohlii to these pathogens (Clemons and Queen). Another study by Abdalrazek demonstrated the essential oil extracted from the plant's tubers, exhibiting anti-microbial properties (Abd El-Razek et al., 2020 ). In agreement with the current study, Malathi study, who concluded that the ethanol extract of Coleus forskohlii root showed the highest antibacterial activity compared with stem and leaf. The antimicrobial activity was observed against Klebsiella pneumonia and Candida albicans in the ethanol extract. (Malathi and Rajkumar, 2015 ). Kanne et al. 's research focused on the root extract of Coleus forskohlii (Kanne et al., 2015 ). In contrast, the current study focused on extracting the effective components from the Leafe part of the plant. Both parts of the plant have antibacterial activity. Conclusion The ethanolic extract of Coleus forskohlii is an effective antibacterial solution. The ethanolic extract was more effective compared to the water extract. Direct application of the ethanolic extract of Coleus forskohlii showed a significant inhibitory antagonism to beta-hemolytic Streptococcus pyogenes, Staphylococcus aureus, E. coli, and many unidentified bacteria grown from plaque samples. Recommendation The present study is a limited experiment but has indicated a potential activity of Coleus forskohlii against gram-positive and gram-negative bacteria. Further experimentation and adjustment to this study by testing more reference and clinical pure cultures could allow getting a better conclusion and further enable in vivo application. Abbreviations ATCC: American Type Culture Collection. PO Box 1549, Manassas, VA 20108 USA. R, resistant; S+, sensitive narrow zone; S++, sensitive medium zone; S+++, sensitive wide zone. ND, not determined. Microorganism Source / Code Ethanolic extract 1:10 Conc. Water extract 1:10 Conc. Positive control (Imipenem 10 μg) Negative control (normal saline) Reference bacteria strains Staphylococcus aureus ATCC 25923 S+ R S+++ R Streptococcus pyogenes Clinical isolate (Pharyngitis) S+ R S+++ R E. coli ATCC 25922 S+++ R Oral clinical samples Sample #1 Clinical isolate (gingivitis) S+ ND ND ND Sample #4 Clinical isolate (gingivitis) S+ ND ND ND Sample #5 Clinical isolate (gingivitis) S+++ ND ND ND Sample #7 Clinical isolate (gingivitis) S+++ ND ND ND Sample #14 Clinical isolate (gingivitis) R ND ND ND Sample #17 Clinical isolate (gingivitis) S+++ ND ND ND Sample #30 Clinical isolate (gingivitis) S+++ ND ND ND. Declarations Ethics approval and consent to participate King Khalid University, Institutional Review Board approved the study and provided the registration number IRB/REG/2022-2023/3. Consent for publication Not applicable Availability of data and materials Not applicable Competing interests The authors declare that they have no competing interests in this study. Funding Not applicable Authors' contributions Taghreed and Hasinah prepared the extract of the plant and set the five solvents. Amel I. and Ghadah performed experimental design and data analysis for antimicrobial activities of different extract concentrations. Aseela and Raida collected the plaque samples and assisted in data analysis and laboratory work, Fadul assisted in laboratory work and preparing the photographs. All authors have read and approved the final manuscript. Acknowledgments Thank all microbiology lab staff at King Khalid University College of Medicines for their support and for carefully taking the study's microbiological work. References ABD EL-RAZEK, S. E., EL-GAMASY, S. M., HASSAN, M., ABDEL-AZIZ, M. S. & NASR, S. M. 2020. Transition metal complexes of a multidentate Schiff base ligand containing guanidine moiety: Synthesis, characterization, anti-cancer effect, and anti-microbial activity. Journal of Molecular Structure, 1203 , 127381. BATISTA, O., SIMÕES, M. F., DUARTE, A., VALDEIRA, M. L., DE LA TORRE, M. C. & RODRÍGUEZ, B. 1995. An antimicrobial abietane from the root of Plectranthus hereroensis. Phytochemistry, 38 , 167-169. BAUER, K., DIETERSDORFER, F., SERTL, K., KAIK, B. & KAIK, G. 1993. Pharmacodynamic effects of inhaled dry powder formulations of fenoterol and colforsin in asthma. Clinical Pharmacology & Therapeutics, 53 , 76-83. BONE, K. 2007. The Ultimate Herbal Compendium: a desktop guide for herbal prescribers , Phytotherapy Press. BREAKPOINTS, C. 2011. CLSI Performance standards for antimicrobial susceptibility testing. Nineteenth informational supplement CLSI document M100-S21 Wayne, PA: Clinical and Laboratory Standards Institute . CAPRIOLI, J., SEARS, M., BAUSHER, L., GREGORY, D. & MEAD, A. 1984. Forskolin lowers intraocular pressure by reducing aqueous inflow. Investigative ophthalmology & visual science, 25 , 268-277. CLEMONS, J. & QUEEN, F. Streptococcus pyogenes. GUO-XIN, M., DAN-YANG, S., XI-ZHOU, G., JUN-CHANG, C., RUI, W., ZHI-GANG, C. & LIANG-AN, C. 2014. Laboratory to clinical investigation of carbapenem resistant Acinetobacter baumannii outbreak in a general hospital. Jundishapur Journal of Microbiology, 7. KANNE, H., BURTE, N. P., PRASANNA, V. & GUJJULA, R. 2015. Extraction and elemental analysis of Coleus forskohlii extract. Pharmacognosy Research, 7 , 237. MAJEED, M. & PRAKASH, S. 2003. Composition and methods containing an antimicrobial essential oil extended from Coleus forskohlii. Google Patents. MALATHI, R. & RAJKUMAR, K. 2015. Phytochemical investigation, GC-MS analysis and in vitro antimicrobial activity of Coleus forskohlii. ||| Bangladesh Journal of Pharmacology, 10 , 924-930. MALLESWARI, D., BAGYANARAYANA, G. & HINDUMATHI, A. 2013. Anti-bacterial activity of Coleus forskohlii extracts against some pathogenic bacteria. J Nat Prod Plant Resour, 3 , 75-78. SHIVAPRASAD, H., PANDIT, S., BHANUMATHY, M., MANOHAR, D., JAIN, V., THANDU, S. A. & SU, X. 2014. Ethnopharmacological and phytomedical knowledge of Coleus forskohlii: an approach towards its safety and therapeutic value. Oriental Pharmacy and Experimental Medicine, 14 , 301-312. Tables Table (1): In-vitro assay of Coleus forskohlii extracts against representatives gram-positive and gram-negative (zone of inhibition in mm). Microorganism Source / Code Ethanolic extract 1:10 Conc Water extract 1:10 Conc Positive control (Imipenem 10µ Negative control (Normal saline) Staphylococcus aureus ATCC 25923 S+ R S+++ R Streptococcus Pyogenes Clinical isolate (Pharyngitis) S+ R S+++ R E. Colli ATCC 25923 S+++ R Oral clinical samples Sample #1 Clinical isolate (gingivitis) S+ ND ND ND Sample #4 Clinical isolate (gingivitis) S+ ND ND ND Sample #5 Clinical isolate (gingivitis) S+++ ND ND ND Sample #7 Clinical isolate (gingivitis) S+++ ND ND ND Sample #14 Clinical isolate (gingivitis) R ND ND ND Sample #17 Clinical isolate (gingivitis) S+++ ND ND ND Sample #30 Clinical isolate (gingivitis) S+++ ND ND ND Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3796502","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":262717855,"identity":"da65d97b-0e23-4400-b4e8-560db9f01a3a","order_by":0,"name":"Amel Ibrahim Faragalla","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA7ElEQVRIiWNgGAWjYHACxgMVILK9B8zj4SNGz4EzIC09QPIAUAsb0VoYJHLAWhgIauGfdvjAgQM19+SZZ749+Phjjp0MGwPzw0c38GiRuJ2WcODAsWLDxtl5yQYHtyUDHcZmbJyDz5rbOQaHP7AlMDbOzjGTOLiNGaiFh00anxb52/kfDhz4l2DfOPMMSEs9YS0Gt3MYDhxsS0hsnMED0nKYsBbD22kGBw72JSQ39uQYG5zddpyHjZmAX+RuJz98cOBbgu3G9jOGDyq3Vdvzszc/fIzX+3DrGmAsZmKUg4A8sQpHwSgYBaNg5AEAEapRIZtWSdEAAAAASUVORK5CYII=","orcid":"","institution":"King Khalid University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Amel","middleName":"Ibrahim","lastName":"Faragalla","suffix":""},{"id":262717856,"identity":"af660e4a-f8ea-4ff7-832b-5d7cc783ddc1","order_by":1,"name":"Ghada Khalid Bahamdan","email":"","orcid":"","institution":"King Khalid University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Ghada","middleName":"Khalid","lastName":"Bahamdan","suffix":""},{"id":262717857,"identity":"e25fe568-10ce-4eca-a484-9703d2bda3d8","order_by":2,"name":"Aseelah Abdullah","email":"","orcid":"","institution":"King Khalid University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Aseelah","middleName":"","lastName":"Abdullah","suffix":""},{"id":262717859,"identity":"268a335b-354a-436d-aef8-083289f8b3f6","order_by":3,"name":"Raida Almoadi","email":"","orcid":"","institution":"King Khalid University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Raida","middleName":"","lastName":"Almoadi","suffix":""},{"id":262717863,"identity":"7fdcc766-0d9b-4f84-a987-106caf70dad7","order_by":4,"name":"Mohammed Fadul","email":"","orcid":"","institution":"King Khalid University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Mohammed","middleName":"","lastName":"Fadul","suffix":""},{"id":262717864,"identity":"5bd8cf86-55ca-4d70-bfb8-e268f27b5ab3","order_by":5,"name":"Tagreed Majrashi","email":"","orcid":"","institution":"King Khalid University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Tagreed","middleName":"","lastName":"Majrashi","suffix":""},{"id":262717865,"identity":"31ac8adf-6416-469d-9138-a9220047e540","order_by":6,"name":"Hasinah Algarni","email":"","orcid":"","institution":"King Khalid University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Hasinah","middleName":"","lastName":"Algarni","suffix":""}],"badges":[],"createdAt":"2023-12-23 13:14:15","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3796502/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3796502/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":48908582,"identity":"481c0813-37a6-4898-9fa2-b1213002d9fe","added_by":"auto","created_at":"2023-12-28 12:16:22","extension":"jpeg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":67253,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cem\u003eSome bacterial strains are susceptible to E. coli (left) and Staphylococcus aureus (right) 0to 1:10 concentration of Coleus forskohlii ethanolic and water extracts. Note the narrow inhibition zone of the ethanolic extract.\u003c/em\u003e\u003c/p\u003e","description":"","filename":"floatimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3796502/v1/a37b7a8bc238db8ee9cc4a43.jpeg"},{"id":48907512,"identity":"67b0bec3-c03c-4241-bc19-b7a4eba46f90","added_by":"auto","created_at":"2023-12-28 12:08:22","extension":"jpeg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":118699,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cem\u003eSusceptibility of Streptococcus pyogenes to 1:10 concentration of Coleus forskohlii ethanolic and water extracts. Note the wide inhibition zone of the ethanolic extract compared to a relatively smaller zone in the case of the water extract.\u003c/em\u003e\u003c/p\u003e","description":"","filename":"floatimage2.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3796502/v1/522204f9e0e3f5cc4ff38887.jpeg"},{"id":48907515,"identity":"b283355f-60b7-46b4-9549-deedd574803e","added_by":"auto","created_at":"2023-12-28 12:08:22","extension":"jpeg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":142892,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cem\u003eDirect susceptibilities of plaque isolate from gingivitis patients to 1:10 concentration of Coleus forskohlii ethanolic and water extracts. Note the narrow inhibition zone of the ethanolic extract. Note the clearance (inhibitions zone) of some plates indicating the collective action of Coleus forskohlii extracts against unidentified isolates (direct method).\u003c/em\u003e\u003c/p\u003e","description":"","filename":"floatimage3.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-3796502/v1/f29b1caa0c8182d09bc7c719.jpeg"},{"id":49110736,"identity":"f3a385fa-27ec-4b1d-b7a3-8fd1704b49e1","added_by":"auto","created_at":"2024-01-03 08:55:08","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":510513,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3796502/v1/0949cc83-3e86-4d43-a4f9-d9c619e6fbbd.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Evaluation of Antimicrobial Activity of Coleus forskohlii on Periodontal Pathogenic Bacteria, An experimental in-vitro study","fulltext":[{"header":"Introduction","content":"\u003cp\u003eMedicinal plants are a rich source of antibacterial agents and curatives. Coleus Forskohlii (C.F) is an important medicinal plant, achievable, non-toxic, and non-irritant used by humans to manage systemic diseases like respiratory and cardiac disorders and skin infections (Bone, 2007). Coleus forskohlii (Lamiaceae), a traditional plant of South India, is a source of bio-reductants and stabilizers (Malleswari et al., 2013). \u0026nbsp;Coleus forskohlii stimulates cyclic adenosine monophosphate, regulates the number of enzymes, and develops some antibacterial effects (Batista et al., 1995). It has biological activities (Caprioli et al., 1984) (Bauer et al., 1993). Some studies reported using C. F. to treat systemic cardiovascular, respiratory disorders, and congestive heart failure (Shivaprasad et al., 2014). It has antimicrobial, antioxidant, and many pharmacological activities (Shivaprasad et al., 2014). The essential oil derived from Coleus forskohlii showed antimicrobial activity against streptococcus species (Majeed and Prakash, 2003). Due to the presence of a wide range of periodontal pathogens and the development of bacterial resistance, the current study tested the antibacterial activity of Coleus Forskohlii against aerobic periodontal pathogens. It aimed to determine the Coleus forskohlii effect on the aerobic bacterial pathogen that initiates gingivitis by collecting plaque samples. However, to our knowledge, no previous study was conducted to test (CF) antibacterial activity against aerobic periodontal pathogens.\u003c/p\u003e"},{"header":"Methods","content":"\u003cp\u003eAn experimental Invitro-study was conducted at the Periodontic Clinic, College of Dentistry, King Khalid University, Abha, Saudi Arabia. This study was approved by the University\u0026apos;s Institutional Review Board and provided the registration number\u0026nbsp;IRB/REG/2022-2023/3.\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003eThe study was according to the ethical principles of the World Medical Association Declaration of Helsinki (2013). Volunteers signed the consent form before participation.\u003c/p\u003e\n\u003cp\u003eThirty plaque samples were collected from male and female gingivitis patients seeking periodontal treatment. A sterile paper point size (40) was inserted in the gingival sulcus for 15 seconds. Then, samples were kept and transported using nutrient broth media suitable for aerobes to the microbiology lab. Coleus forskohlii leaves were collected from the Aseer region, Abha Kingdom of Saudi Arabia, in October 2022. The leaves were carefully rinsed with distilled water, dried overnight in a hot air oven at 40\u0026deg;C, and ground to a particle size of 40 mesh by using mortar and pestle. The plant powder (32.6 g) was divided into five portions, and each portion was extracted with a solvent, including water, methanol, ethyl acetate, chloroform, and hexane by decoction for two hours, followed by maceration for 48 hours to get five extracts.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAssay for the antimicrobial activity of Coleus forskohlii extract\u0026nbsp;\u003c/strong\u003ewas done by the disk impregnation method with minor modification from the following standard methods (Breakpoints, 2011) : One to three loopful of 24 hold cultures from each test strain were used to prepare 0.5 McFarland standard suspensions. A loopful of 0.5 McFarland suspensions was used to streak Mueller Hinton\u0026rsquo;s agar plates (Difco). Discs were placed on the inoculated agar, and inoculated plates were incubated at 37\u0026deg;C and examined after 24, 48, and 72 hours to detect the inhibition zones. Inhibition zones were measured in mm by a ruler for each bacterial strain under and around discs. The minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) have been considered valuable endpoints of antibacterial activity to guide effective therapy of bacterial infections. The disc impregnated discs at various concentrations have been tested to determine the MIC.\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003eThe antimicrobial activity of ethanolic and water extract of Coleus forskohlii against clinical and reference bacterial strains is shown in Table 1 and Figures 1, 2, and 3. Isolation and the preliminary naming standard were performed as per routine laboratory methods and following standard protocols\u0026nbsp;(Guo-Xin et al., 2014). The identity of isolates was confirmed using the Vitek 2 automated system according to manufacturer procedures (bio M\u0026eacute;rieux VITEK, Inc.). Vitek 2 was also used to test their antimicrobial sensitivities.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe ethanolic and water extracts showed different activities against the selected bacteria, but the ethanolic extract was superior to the water extract. A wide spectrum inhibitory activity was shown against beta-hemolytic Streptococcus pyogenes, an isolate from plaque samples. The activity against Staphylococcus aureus and E. coli was less than that against Streptococcus pyogenes. The direct in-vitro assay was applied to 10 clinical samples grown on Tryptocase Soy broth. The grown cultures were tested directly against the ethanolic extract of Coleus forskohlii. Various activities were demonstrated, indicating the positive inhibitory reactions of Coleus forskohlii ethanolic extracts against many bacteria grown directly from plaque samples. Direct application of an ethanolic extract of Coleus forskohlii indicated a good inhibitory antagonism to many unidentified bacteria grown from plaque samples. Twenty-five bacteria have been inhibited at a concentration of 1g / mL. Most (82.9%) of the organisms have been inhibited at 1g / mL, whereas 34.3% were inhibited at 0.25 g/ mL, and only 5.7% were inhabited at 0.15 g/ mL; the latter two concentrations represented the MIC for most of the strains. Table 1. In vitro assay of Coleus forskohlii extract against representatives gram-positive and gram-negative (zone of inhibition in mm). Figure 1. Some bacterial strains are susceptible to E. coli (left) and Staphylococcus aureus (right) to 1:10 concentration of Coleus forskohlii ethanolic and water extracts. Note the narrow inhibition zone of the ethanolic extract. Figure 2. Showed Susceptibility of Streptococcus pyogenes to 1:10 concentration of Coleus forskohlii ethanolic and water extracts. There was a wide inhibition zone of the ethanolic extract compared to a relatively smaller zone in the case of the water extract. Figure 3. Showed direct susceptibilities of isolates from patient gingivitis to 1:10 concentration of Coleus forskohlii ethanolic and water extracts. Note the narrow inhibition zone of the ethanolic extract. Note the clearance (inhibitions zone) of some plates indicating the collective action of Coleus forskohlii extracts against unidentified isolates (direct method).\u0026nbsp;\u003c/p\u003e"},{"header":"Discussions","content":"\u003cp\u003eIn the current study, five extracts of Coleus forskohlii were tested for antimicrobial activity against \u003cem\u003ebeta-hemolytic Streptococcus pyogenes\u003c/em\u003e, a Gram-positive, aerotolerant bacteria in the genus \u003cem\u003eStreptococcus\u003c/em\u003e. These bacteria are extracellular and comprise non-motile and non-sporing cocci (round cells) that tend to link in chains. They are clinically important for humans, as they are infrequent but usually pathogenic (Clemons and Queen). \u003cem\u003eE. coli and Staphylococcus aureus\u003c/em\u003e all were isolated from plaque samples. Assessed by the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC). Ethanolic and water extracts showed a significant wide-spectrum inhibitory activity and reduction of the tested organism.\u003c/p\u003e \u003cp\u003eIn agreement with this study, Pullaiah tested the activity of Coleus forskohlii in an oil extract against \u003cem\u003eStaphylococcus aureus, E. coli Staphylococcus aureus, streptococcus species\u003c/em\u003e, and other aerobic bacterial pathogens and concluded with the presence of antibacterial activity of Coleus forskohlii to these pathogens (Clemons and Queen). Another study by Abdalrazek demonstrated the essential oil extracted from the plant's tubers, exhibiting anti-microbial properties (Abd El-Razek et al., \u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e2020\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eIn agreement with the current study, Malathi study, who concluded that the ethanol extract of Coleus forskohlii root showed the highest antibacterial activity compared with stem and leaf. The antimicrobial activity was observed against \u003cem\u003eKlebsiella pneumonia\u003c/em\u003e and \u003cem\u003eCandida albicans\u003c/em\u003e in the ethanol extract. (Malathi and Rajkumar, \u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e2015\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eKanne et al. 's research focused on the root extract of Coleus forskohlii (Kanne et al., \u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e2015\u003c/span\u003e). In contrast, the current study focused on extracting the effective components from the Leafe part of the plant. Both parts of the plant have antibacterial activity.\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003eThe ethanolic extract of Coleus forskohlii is an effective antibacterial solution. The ethanolic extract was more effective compared to the water extract. Direct application of the ethanolic extract of Coleus forskohlii showed a significant inhibitory antagonism to beta-hemolytic Streptococcus pyogenes, Staphylococcus aureus, E. coli, and many unidentified bacteria grown from plaque samples.\u003c/p\u003e"},{"header":"Recommendation","content":"\u003cp\u003eThe present study is a limited experiment but has indicated a potential activity of Coleus forskohlii against gram-positive and gram-negative bacteria.\u003c/p\u003e \u003cp\u003eFurther experimentation and adjustment to this study by testing more reference and clinical pure cultures could allow getting a better conclusion and further enable in vivo application.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003eATCC: \u0026nbsp;American Type Culture Collection. PO Box 1549, Manassas, VA 20108 USA. R, resistant; S+, sensitive narrow zone; S++, sensitive medium zone; S+++, sensitive wide zone. ND, not determined. Microorganism Source / Code Ethanolic extract 1:10 Conc. Water extract 1:10 Conc. Positive control (Imipenem 10 \u0026mu;g) Negative control (normal saline) Reference bacteria strains Staphylococcus aureus ATCC 25923 S+ R S+++ R Streptococcus pyogenes Clinical isolate (Pharyngitis) S+ R S+++ R E. coli ATCC 25922 S+++ R Oral clinical samples Sample #1 Clinical isolate (gingivitis) S+ ND ND ND Sample #4 Clinical isolate (gingivitis) S+ ND ND ND Sample #5 Clinical isolate (gingivitis) S+++ ND ND ND Sample #7 Clinical isolate (gingivitis) S+++ ND ND ND Sample #14 Clinical isolate (gingivitis) R ND ND ND Sample #17 Clinical isolate (gingivitis) S+++ ND ND ND Sample #30 Clinical isolate (gingivitis) S+++ ND ND ND.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eKing Khalid University, Institutional Review Board approved the study\u0026nbsp;and provided the registration number\u0026nbsp;IRB/REG/2022-2023/3.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare that they have no competing interests in this study.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026apos; contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eTaghreed and Hasinah prepared the extract of the plant and set the five solvents. Amel I. and Ghadah performed experimental design and data analysis for antimicrobial activities of different extract concentrations. Aseela and Raida collected the plaque samples and assisted in data analysis and laboratory work, Fadul assisted in laboratory work and preparing the photographs. All authors have read and approved the final manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgments\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThank all microbiology lab staff at King Khalid University College of Medicines for their support and for carefully taking the study\u0026apos;s microbiological work.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eABD EL-RAZEK, S. E., EL-GAMASY, S. M., HASSAN, M., ABDEL-AZIZ, M. S. \u0026amp; NASR, S. M. 2020. Transition metal complexes of a multidentate Schiff base ligand containing guanidine moiety: Synthesis, characterization, anti-cancer effect, and anti-microbial activity. \u003cem\u003eJournal of Molecular Structure,\u003c/em\u003e 1203\u003cstrong\u003e,\u003c/strong\u003e 127381.\u003c/li\u003e\n\u003cli\u003eBATISTA, O., SIM\u0026Otilde;ES, M. F., DUARTE, A., VALDEIRA, M. L., DE LA TORRE, M. C. \u0026amp; RODR\u0026Iacute;GUEZ, B. 1995. An antimicrobial abietane from the root of Plectranthus hereroensis. \u003cem\u003ePhytochemistry,\u003c/em\u003e 38\u003cstrong\u003e,\u003c/strong\u003e 167-169.\u003c/li\u003e\n\u003cli\u003eBAUER, K., DIETERSDORFER, F., SERTL, K., KAIK, B. \u0026amp; KAIK, G. 1993. Pharmacodynamic effects of inhaled dry powder formulations of fenoterol and colforsin in asthma. \u003cem\u003eClinical Pharmacology \u0026amp; Therapeutics,\u003c/em\u003e 53\u003cstrong\u003e,\u003c/strong\u003e 76-83.\u003c/li\u003e\n\u003cli\u003eBONE, K. 2007. \u003cem\u003eThe Ultimate Herbal Compendium: a desktop guide for herbal prescribers\u003c/em\u003e, Phytotherapy Press.\u003c/li\u003e\n\u003cli\u003eBREAKPOINTS, C. 2011. CLSI Performance standards for antimicrobial susceptibility testing. \u003cem\u003eNineteenth informational supplement CLSI document M100-S21 Wayne, PA: Clinical and Laboratory Standards Institute\u003c/em\u003e.\u003c/li\u003e\n\u003cli\u003eCAPRIOLI, J., SEARS, M., BAUSHER, L., GREGORY, D. \u0026amp; MEAD, A. 1984. Forskolin lowers intraocular pressure by reducing aqueous inflow. \u003cem\u003eInvestigative ophthalmology \u0026amp; visual science,\u003c/em\u003e 25\u003cstrong\u003e,\u003c/strong\u003e 268-277.\u003c/li\u003e\n\u003cli\u003eCLEMONS, J. \u0026amp; QUEEN, F. Streptococcus pyogenes.\u003c/li\u003e\n\u003cli\u003eGUO-XIN, M., DAN-YANG, S., XI-ZHOU, G., JUN-CHANG, C., RUI, W., ZHI-GANG, C. \u0026amp; LIANG-AN, C. 2014. Laboratory to clinical investigation of carbapenem resistant Acinetobacter baumannii outbreak in a general hospital. \u003cem\u003eJundishapur Journal of Microbiology,\u003c/em\u003e 7.\u003c/li\u003e\n\u003cli\u003eKANNE, H., BURTE, N. P., PRASANNA, V. \u0026amp; GUJJULA, R. 2015. Extraction and elemental analysis of Coleus forskohlii extract. \u003cem\u003ePharmacognosy Research,\u003c/em\u003e 7\u003cstrong\u003e,\u003c/strong\u003e 237.\u003c/li\u003e\n\u003cli\u003eMAJEED, M. \u0026amp; PRAKASH, S. 2003. Composition and methods containing an antimicrobial essential oil extended from Coleus forskohlii. Google Patents.\u003c/li\u003e\n\u003cli\u003eMALATHI, R. \u0026amp; RAJKUMAR, K. 2015. Phytochemical investigation, GC-MS analysis and in vitro antimicrobial activity of Coleus forskohlii. \u003cem\u003e||| Bangladesh Journal of Pharmacology,\u003c/em\u003e 10\u003cstrong\u003e,\u003c/strong\u003e 924-930.\u003c/li\u003e\n\u003cli\u003eMALLESWARI, D., BAGYANARAYANA, G. \u0026amp; HINDUMATHI, A. 2013. Anti-bacterial activity of Coleus forskohlii extracts against some pathogenic bacteria. \u003cem\u003eJ Nat Prod Plant Resour,\u003c/em\u003e 3\u003cstrong\u003e,\u003c/strong\u003e 75-78.\u003c/li\u003e\n\u003cli\u003eSHIVAPRASAD, H., PANDIT, S., BHANUMATHY, M., MANOHAR, D., JAIN, V., THANDU, S. A. \u0026amp; SU, X. 2014. Ethnopharmacological and phytomedical knowledge of Coleus forskohlii: an approach towards its safety and therapeutic value. \u003cem\u003eOriental Pharmacy and Experimental Medicine,\u003c/em\u003e 14\u003cstrong\u003e,\u003c/strong\u003e 301-312.\u003c/li\u003e\n\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp\u003e\u003cstrong\u003eTable (1):\u003c/strong\u003e In-vitro assay of Coleus forskohlii extracts against representatives gram-positive and gram-negative (zone of inhibition in mm).\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"643\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eMicroorganism\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eSource / Code\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003eEthanolic extract\u003c/p\u003e\n \u003cp\u003e1:10 Conc\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003eWater extract\u003c/p\u003e\n \u003cp\u003e1:10 Conc\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003ePositive control (Imipenem 10\u0026micro;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eNegative control (Normal saline)\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eStaphylococcus aureus\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eATCC\u0026nbsp;25923\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003eS+\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003eS+++\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eStreptococcus Pyogenes\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eClinical\u0026nbsp;isolate\u0026nbsp;(Pharyngitis)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003eS+\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003eS+++\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eE. Colli\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eATCC\u0026nbsp;25923\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003eS+++\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"6\" valign=\"top\"\u003e\n \u003cp\u003eOral clinical samples\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eSample #1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eClinical\u0026nbsp;isolate\u0026nbsp;(gingivitis)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003eS+\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eSample #4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eClinical\u0026nbsp;isolate\u0026nbsp;(gingivitis)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003eS+\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eSample #5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eClinical\u0026nbsp;isolate\u0026nbsp;(gingivitis)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003eS+++\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eSample #7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eClinical\u0026nbsp;isolate\u0026nbsp;(gingivitis)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003eS+++\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eSample #14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eClinical\u0026nbsp;isolate\u0026nbsp;(gingivitis)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003eR\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eSample #17\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eClinical\u0026nbsp;isolate\u0026nbsp;(gingivitis)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003eS+++\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"15.217391304347826%\" valign=\"top\"\u003e\n \u003cp\u003eSample #30\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.993788819875776%\" valign=\"top\"\u003e\n \u003cp\u003eClinical\u0026nbsp;isolate\u0026nbsp;(gingivitis)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"17.70186335403727%\" valign=\"top\"\u003e\n \u003cp\u003eS+++\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.838509316770187%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"18.322981366459626%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"16.925465838509318%\" valign=\"top\"\u003e\n \u003cp\u003eND\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Aerobic pathogens, (Bone, 2007): Coleus forskohlii, Ethanol, Gingivitis, Plant extract","lastPublishedDoi":"10.21203/rs.3.rs-3796502/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3796502/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground:\u003c/h2\u003e \u003cp\u003eColeus Forskohlii (C.F.) is a typical indigenous medicinal plant from the Lamiaceae family grown widely in India. Coleus gained importance after discovering its therapeutic properties (Bone, \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e2007\u003c/span\u003e) and was reported to have some antibacterial activity. The current study tested the antibacterial effect of (C F) on certain periodontal pathogens.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eAn experimental Invitro-study was conducted at King Khalid University, to test the antibacterial activity of Coleus Forskohlii. Thirty plaque samples were collected from gingivitis patients, kept, and transported using nutrient broth to the microbiology lab. The (CF) extract was prepared from the plant's leaves and five solutions were produced (water, methanol, ethyl acetate, chloroform, and hexane). Following the bacterial culturing, the extract solutions were applied and the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) were measured.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eVarious activities were demonstrated, indicating the positive inhibitory reactions of ethanolic extracts against many bacteria grown directly from plaque samples. Twenty-five bacterial species have been inhibited at 1g / mL concentrations. Most organisms (82.9%) have been inhibited at 1g / mL, whereas 34.3% were inhibited at 0.25 g/ mL, and only 5.7% were inhabited at 0.15 g/ mL; the latter two concentrations represented the MIC for most of the strains.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e \u003cp\u003eThe ethanolic extract of Coleus forskohlii demonstrated effective antimicrobial activity. The ethanolic extract and water extracts showed different activities against the selected bacteria, but the ethanolic extract was superior to the water extract.\u003c/p\u003e","manuscriptTitle":"Evaluation of Antimicrobial Activity of Coleus forskohlii on Periodontal Pathogenic Bacteria, An experimental in-vitro study","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2023-12-28 12:08:17","doi":"10.21203/rs.3.rs-3796502/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"3e47ee05-b8d0-44d2-8733-7a11dc1faba6","owner":[],"postedDate":"December 28th, 2023","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-01-29T01:59:16+00:00","versionOfRecord":[],"versionCreatedAt":"2023-12-28 12:08:17","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-3796502","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3796502","identity":"rs-3796502","version":["v1"]},"buildId":"cBFmMYwuxLRRLfASyISRj","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}