CSDC2, an RBP essential to cardiomyocyte commitment during cardiac differentiation in hiPSC

2026 · doi:10.64898/2026.02.12.705579
preprint OA: closed
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This study investigated the RNA-binding protein CSDC2 during cardiomyocyte differentiation from human pluripotent stem cells, focusing on its role at the final stages of cardiac lineage commitment. Using CSDC2 loss-of-function in the differentiation process, the authors found that CSDC2 knockout impairs cardiomyocyte differentiation, while restoring CSDC2 expression rescues differentiation potential. They used polysome profiling to characterize CSDC2-dependent changes in the translatome during cardiac differentiation and reported that in cardiac mesoderm cells CSDC2 interacts with ribosomal proteins and associates with mRNAs encoding regulators of cardiac progenitor commitment. The work is limited to the hiPSC cardiac differentiation model and does not address other cell types or in vivo contexts. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

Cardiovascular diseases are the leading cause of death worldwide, accounting for approximately 30% of total mortality. Changes in post-transcriptional regulation have been correlated with the development of cardiopathies. RNA-binding proteins (RBP) are proteins capable of interacting with mRNAs, regulating their stability, localization, and translation. Here, we described CSDC2 as an RBP expressed at the final stages of cardiac differentiation using hPSCs as a model. We showed that the loss of CSDC2 impairs cardiomyocyte differentiation, while the recovery of its expression rescues the differentiation potential of these cells. We characterized the translatome of CSDC2 knockout cells during cardiac differentiation by polysome profiling. In cardiac mesoderm cells, CSDC2 interacts with ribosomal proteins. Furthermore, CSDC2 appears to be able to associate with mRNAs encoding regulators of cardiac progenitor commitment. Altogether, in this study, we describe a new role of CSDC2 in cardiomyocyte commitment using cardiac differentiation of hiPSCs.
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Abstract Cardiovascular diseases are the leading cause of death worldwide, accounting for approximately 30% of total mortality. Changes in post-transcriptional regulation have been correlated with the development of cardiopathies. RNA-binding proteins (RBP) are proteins capable of interacting with mRNAs, regulating their stability, localization, and translation. Here, we described CSDC2 as an RBP expressed at the final stages of cardiac differentiation using hPSCs as a model. We showed that the loss of CSDC2 impairs cardiomyocyte differentiation, while the recovery of its expression rescues the differentiation potential of these cells. We characterized the translatome of CSDC2 knockout cells during cardiac differentiation by polysome profiling. In cardiac mesoderm cells, CSDC2 interacts with ribosomal proteins. Furthermore, CSDC2 appears to be able to associate with mRNAs encoding regulators of cardiac progenitor commitment. Altogether, in this study, we describe a new role of CSDC2 in cardiomyocyte commitment using cardiac differentiation of hiPSCs. Competing Interest Statement The authors have declared no competing interest.

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