An extraction-free method for rapid detection of six respiratory pathogens in one tube using polychromatic melting curve analysis

Respiratory viruses like 2019 - nCoV, influenza viruses, respiratory syncytial virus, adenoviruses, and Mycoplasma Pneumonia are highly epidemic. Detecting these six is crucial for disease control. We designed specific primers and probes and developed an extraction - free method based on fluorescence PCR melting curve for sensitive detection. It has high sensitivity (single - copy detection limit), can detect six viruses simultaneously by one - step amplification for multiplex detection.