On the complementation of Saccharomyces cerevisiae MET16 in Schizosaccharomyces pombe

Metabolically active pathways tend to be more highly conserved between ecologically similar relatives than other pathways for their critical role in life-functions. The developmental features of Schizosaccharomyces pombe and Saccharomyces cerevisiae may have become quite divergent, but their ecological niche is still quite similar. The functional homology between S. cerevisiae’s MET16p and S. pombe’s was investigated through plasmid insertion and sulfate assimilation assays in S. pombe. Although the S. cerevisiae:MET16p was produced, the S. pombe colonies were unable to utilize extracellular sulfate in the synthesis of Methionine.