A high-resolution brain proteome map uncovers the Inter-hemispheric laterality & Inter-regional protein expression changes

Abstract The human brain has always been a black box full of mysteries. Here we present one of the most comprehensive proteomics investigation of the brain, focusing on inter-hemispheric differences. An extensive mass spectrometry-based analysis of 19 brain regions from both left and right hemispheres measured more than 3300 proteins and 38700 peptides. This high-resolution data provides a comprehensive coverage of experimentally measured (non-hypothetical) proteins across various regions to characterize inter-hemispheric differences. We also tried to understand the brain proteins in terms of synapse analysis. The study has attempted to investigate the expression of neuroanatomical allied region and brain disorder protein markers in 19 region and sub-region of brain. Furthermore, we have developed the most comprehensive Brain Proteome Database, based on our, and publicly available curated data representing more than 9000 proteins (with isoforms) and around 90000 peptides at www.brainprot.org, which can aid in understanding the human brain’s complexity.