Expression of lncRNA MSC-AS1 in hepatocellular carcinoma cell lines and its effect on proliferation, apoptosis, and migration.

Kou X, Zhu J, Xie X, Hao M, Zhao Y
The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2020 · doi:10.5152/tjg.2020.19485 · PMID:33625998 · PMC7928261
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This study investigated the role of long non-coding RNA MSC-AS1 in hepatocellular carcinoma by analyzing its expression across several HCC cell lines and a normal hepatocyte line. The researchers employed siRNA-mediated knockdown to assess the impact of reduced MSC-AS1 levels on cellular behaviors, including proliferation, apoptosis, migration, and invasion. Results indicated that MSC-AS1 is significantly upregulated in cancer cells, and its suppression effectively inhibited tumor growth and spread while promoting cell death. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

Background/aimsThis study aimed to explore the expression of long non-coding RNA MSC-AS1 in hepatocellular carcinoma (HCC) cells and its effect on the proliferation, migration, and apoptosis of HCC cells.Materials and methodsThe expression of MSC-AS1 in HCC cell lines BEL7402, SMMC7721, Huh7, HepG2, MHCC97-H, and normal hepatocyte line L02 was detected by reverse transcriptase polymerase chain reaction. The HCC cells were divided into blank, negative control (NC)-small interfering RNA (siRNA) (transfected with negative siRNA), and MSC-AS1 siRNA (transfected with MSC-AS1 siRNA) groups. Cell counting kit-8 and colony formation assays were used to determine the proliferation, and cell apoptosis, migration, and invasion were detected by flow cytometry, wound healing, and transwell assays, respectively. Western blot was used to detect the expression of related proteins.ResultsThe expression of MSC-AS1 in HCC cell lines was significantly higher than that in L02. In the MSC-AS1 siRNA group, the proliferation and colony formation of HCC cells were inhibited, whereas the apoptosis rate was significantly higher than that in the blank and NC-siRNA groups. The rate of wound healing and the number of invasion cells in the MSC-AS1 siRNA group were significantly lower than that in the blank and NC-siRNA groups.ConclusionMSC-AS1 was upregulated in HCC cells, and the downregulation of MSC-AS1 could inhibit cell proliferation, migration, and invasion and promote apoptosis of HCC cells.
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Abstract

Background/Aims: This study aimed to explore the expression of long non-coding RNA MSC-AS1 in hepatocellular carcinoma (HCC) cells and its effect on the proliferation, migration, and apoptosis of HCC cells.

Materials and methods

The expression of MSC-AS1 in HCC cell lines BEL7402, SMMC7721, Huh7, HepG2, MHCC97-H, and normal hepatocyte line L02 was detected by reverse transcriptase polymerase chain reaction. The HCC cells were divided into blank, negative control (NC)–small interfering RNA (siRNA) (transfected with negative siRNA), and MSC-AS1 siRNA (transfected with MSC-AS1 siRNA) groups. Cell counting kit-8 and colony formation assays were used to determine the proliferation, and cell apoptosis, migration, and invasion were detected by flow cytometry, wound healing, and transwell assays, respectively. Western blot was used to detect the expression of related proteins.

Results

The expression of MSC-AS1 in HCC cell lines was significantly higher than that in L02. In the MSC-AS1 siRNA group, the proliferation and colony formation of HCC cells were inhibited, whereas the apoptosis rate was significantly higher than that in the blank and NC-siRNA groups. The rate of wound healing and the number of invasion cells in the MSC-AS1 siRNA group were significantly lower than that in the blank and NC-siRNA groups.

Conclusion

MSC-AS1 was upregulated in HCC cells, and the downregulation of MSC-AS1 could inhibit cell proliferation, migration, and invasion and promote apoptosis of HCC cells. Article Info Published In Journal Turkish Journal of Gastroenterology Volume / Issue Volume 31 · Issue 12 Pages 860-867 History Published Online December 15, 2020 Copyright Copyright (c) 2020 Xiaoni Kou, Jiang Zhu, Xinke Xie, Mingxia Hao, Yingren Zhao Affiliations Xiaoni Kou Department of infectious diseases The First Affiliated Hospital of Xi?an Jiaotong University Xi?an Shaanxi Province China Jiang Zhu Department of Galactophore, Shaanxi Provincial Cancer Hospital, Shaanxi, China Xinke Xie Department of Hepatopathy, Shaanxi University of Chinese Medicine Affiliated Hospital, Shaanxi, China Mingxia Hao Department of Hepatopathy, Shaanxi University of Chinese Medicine Affiliated Hospital, Shaanxi, China Yingren Zhao Department of Infectious Diseases, The First Affiliated Hospital of Xi?an Jiaotong University, Shaanxi, China Cite this Article Kou, X., Zhu, J., Xie, X., Hao, M., & Zhao, Y. (2020). Expression of lncRNA MSC-AS1 in hepatocellular carcinoma cell lines and its effect on proliferation, apoptosis, and migration. Turkish Journal of Gastroenterology, 31(12), 860–867. https://doi.org/10.5152/tjg.2020.19485 Outlines

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