A protocol to generate SPH-OminiCMV-Ents mESCs

Abstract Detection of genetic information in the natural context is essential to understand the biological principles. However, the ability to sense the activity of endogenous genes is limited for conventional tools. Here, we developed a highly programmable sgRNA switch system (Ents) that enables detection of endogenous genes and lncRNAs, even with very low expression level. This protocol is related to the publication “Endogenous promoter-driven sgRNA for monitoring the expression of low-abundant genes and lncRNAs” in Nature Cell Biology.