Ampule-sealed Acidolysis for Monosaccharide Composition Analysis of Serum or Plasma Samples
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CC-BY-4.0
Abstract
Abstract This protocol describes the procedures where an ampule-sealed acidolysis is used for releasing glycan monosaccharides from serum or plasma samples. The monosaccharide composition analysis is subsequently obtained by a traditional HPLC method that separates and quantifies all 1-phenyl-3-methyl-5-pyrazolone (PMP)-labeled monosaccharides in 10 μl serum in 55 minutes. The described workflow takes approximately 2 days, up to 26 serum samples can be analyzed with one HPLC instrument per day. Each step of the experimental procedures has been optimized with repeated tests of monosaccharide standards and serum samples.
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Source provenance
- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00
- unpaywall
- last seen: 2026-05-28T02:00:01.590549+00:00
License: CC-BY-4.0