An Ultra-rapid and Green Method for Simultaneous Determination of Honokiol and Magnolol in Magnoliae Officinalis Cortex with One Standard by HPLC-UV at Equal Absorption Wavelength

Abstract An ultra-rapid and green assay method for simultaneous determination of honokiol and magnolol in Magnoliae Officinalis Cortex with one standard was developed by HPLC-UV at equal absorption wavelength. The sample was prepared by ultrasonic-assisted matrix solid-phase dispersion. The HPLC separation was performed on a Poroshell C18 column with an eco-friendly mobile phase. The detection wavelength was set at the equal absolution wavelength of honokiol and magnolol (247 nm). The contents of honokiol and magnolol in six batches of samples, obtained by developed method with one marker and external standard method with two markers, were comparable. In addition, the developed HPLC method only took 2.5 min and 4.55 mL green organic solution (ethanol), which including the sample extraction and separation. The developed method was rapid, green and standard saving, which would be helpful to improve the quality evaluation of Magnoliae Officinalis Cortex.