Biodegradation of PAHs in drilling wastes from oil-contaminated soil using an isolated halotolerant bacterial consortium

Biodegradation of PAHs in drilling wastes from oil-contaminated soil using an isolated halotolerant bacterial consortium | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Biodegradation of PAHs in drilling wastes from oil-contaminated soil using an isolated halotolerant bacterial consortium Neamatollah Jaafarzadeh, Nastaran Talepour, Effat Abbasi Montazeri, and 11 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4982779/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Petroleum contaminants and fossil fuel activities contribute significantly to environmental pollution, especially in regions involved in oil production. Polyaromatic hydrocarbons (PAHs) in petroleum products are hazardous to the environment and human health because of their intricate chemical makeup and cancer-causing characteristics. In this study, we isolated and characterized bacterial strains from oil-contaminated soil using 16S rRNA analysis to evaluate their potential for degrading PAHs commonly found in drilling wastes. We also analyzed the PAH content in drilling cuttings and assessed the effectiveness of PAH biodegradation by the isolated bacterial consortium on real oil-drilled cutting samples. Additionally, a bioremediation consortium comprising Pseudomonas stutzeri , Pseudomonas aeruginosa , and Bacillus subtilis was used to remediate PAHs. This study suggests that indigenous bacteria contribute favorably to the process of PAH biodegradation. We observed that benzo(a)anthracene exhibited the fastest degradation rate among the tested compounds, while fluorene showed slower degradation than the other tested compounds. Importantly, the efficiency of all tested scenarios surpassed 97%, highlighting the efficacy of the bioremediation approach in reducing PAH contamination. These results underscore the potential of utilizing bacterial consortia for effective PAH remediation, contributing to the development of sustainable strategies for environmental remediation. Biodegradation Bacterial consortium Halotolerant bacteria Oil-drilled cutting PAHs Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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