Lignin Structural Remodeling during Biodegradation of Oil Palm Empty Fruit Bunch by Trametes hirsuta EDN082

Lignin Structural Remodeling during Biodegradation of Oil Palm Empty Fruit Bunch by Trametes hirsuta EDN082 | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Lignin Structural Remodeling during Biodegradation of Oil Palm Empty Fruit Bunch by Trametes hirsuta EDN082 Fitria Maulita, Anja Meryandini, Dede Heri Yuli Yanto, Sri Listiyowati, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8846722/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Oil palm empty fruit bunches (OPEFB) are lignocellulosic biomass that can be used for bioethanol and biopulping. The conversion of lignocellulosic biomass is needed to break the bonds of lignin so cellulose can be used for bioethanol production. The lignin bond breaking was biologically selected using the white rot fungus Trametes hirsuta EDN 082. This study aimed to compare and analyze the structure of lignin resulting from the biological degradation of lignin by the white rot fungus T. hirsuta EDN 082 combined with chemical pretreatment. The substrate used was OPEFB with a size of 40-60 mesh with various control treatments (C), microwave heating-water (W), microwave heating-oxalic acid (OA), C and T. hirsuta (JC), W and T. hirsuta (JW), OA and T. hirsuta (JOA). The substrate added by T. hirsuta was incubated for 21 days. Chemical analysis was conducted to determine weight loss, lignin content, and cellulose from various treatments. From the six treatment variations, it was shown that the highest average loss of lignin (85.92%) with the loss of α-cellulose (79.61%) and hemicellulose (80.15%) occurred in JC culture. This is supported by the change in the functional group band at 1161-1645 cm-1. Keywords: Lignin degradation, White-rot fungi, Lignocellulosic biomass Lignin degradation White-rot fungi Lignocellulosic biomass Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8846722","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":594219999,"identity":"cdab0af1-b570-4bc7-9100-075e9f1fd182","order_by":0,"name":"Fitria Maulita","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAzklEQVRIiWNgGAWjYHACxgMJDAxy/CBmQgGReg4kJDAYSzaAtBgQq4UhgSFxwwEQkxgtuu1nDA48/GFnbHx+deKHBwYM8vxiB/BrMTuTYwB0WLKc2Y23myWADjOcOTuBgJYDYC3MxmY3zm4AaUkwuE1Iy/k3IC31iZtnnN38gzgtN8C2HE7cwN+7jUhbbjwrOJCQdtxY4gbvNosEAwki/HI+eePDHzbVcvz9Zzff/FFhI88vTUALAkiAVUoQqxwE+A+QonoUjIJRMApGEgAA/kRK+CEkUgcAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0003-0516-703X","institution":"IPB University: Institut Pertanian Bogor","correspondingAuthor":true,"prefix":"","firstName":"Fitria","middleName":"","lastName":"Maulita","suffix":""},{"id":594220000,"identity":"92b62517-7ad8-4d51-a06c-e8d3a12821e5","order_by":1,"name":"Anja Meryandini","email":"","orcid":"","institution":"IPB University: Institut Pertanian Bogor","correspondingAuthor":false,"prefix":"","firstName":"Anja","middleName":"","lastName":"Meryandini","suffix":""},{"id":594220001,"identity":"f6443f43-e40f-45cd-9607-5a5dc5313030","order_by":2,"name":"Dede Heri Yuli Yanto","email":"","orcid":"","institution":"LIPI: Lembaga Ilmu Pengetahuan Indonesia","correspondingAuthor":false,"prefix":"","firstName":"Dede","middleName":"Heri Yuli","lastName":"Yanto","suffix":""},{"id":594220002,"identity":"b698fff0-4053-416e-ab35-2c6360a967c9","order_by":3,"name":"Sri Listiyowati","email":"","orcid":"","institution":"IPB: Institut Pertanian Bogor","correspondingAuthor":false,"prefix":"","firstName":"Sri","middleName":"","lastName":"Listiyowati","suffix":""},{"id":594220003,"identity":"07993707-531f-44d4-b60a-2298ea5357de","order_by":4,"name":"Sita Heris Anita","email":"","orcid":"","institution":"LIPI: Lembaga Ilmu Pengetahuan Indonesia","correspondingAuthor":false,"prefix":"","firstName":"Sita","middleName":"Heris","lastName":"Anita","suffix":""},{"id":594220004,"identity":"6d74197c-b0bb-452d-8c48-21d1d0eef9f4","order_by":5,"name":"Maulida Oktaviani","email":"","orcid":"","institution":"LIPI: Lembaga Ilmu Pengetahuan Indonesia","correspondingAuthor":false,"prefix":"","firstName":"Maulida","middleName":"","lastName":"Oktaviani","suffix":""}],"badges":[],"createdAt":"2026-02-11 03:43:29","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-8846722/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8846722/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":107869374,"identity":"d4d2f141-bfda-4959-8e0e-ae4abffacc58","added_by":"auto","created_at":"2026-04-27 07:36:54","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":609578,"visible":true,"origin":"","legend":"","description":"","filename":"2.MaulitaetalAppliedBiochemBiotechSpringer.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8846722/v1_covered_fa5a4f0f-3a53-4fab-95b0-565e16b6d0e4.pdf"}],"financialInterests":"","formattedTitle":"Lignin Structural Remodeling during Biodegradation of Oil Palm Empty Fruit Bunch by Trametes hirsuta EDN082","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Lignin degradation, White-rot fungi, Lignocellulosic biomass","lastPublishedDoi":"10.21203/rs.3.rs-8846722/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8846722/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Oil palm empty fruit bunches (OPEFB) are lignocellulosic biomass that can be used for bioethanol and biopulping. The conversion of lignocellulosic biomass is needed to break the bonds of lignin so cellulose can be used for bioethanol production. The lignin bond breaking was biologically selected using the white rot fungus Trametes hirsuta EDN 082. This study aimed to compare and analyze the structure of lignin resulting from the biological degradation of lignin by the white rot fungus T. hirsuta EDN 082 combined with chemical pretreatment. The substrate used was OPEFB with a size of 40-60 mesh with various control treatments (C), microwave heating-water (W), microwave heating-oxalic acid (OA), C and T. hirsuta (JC), W and T. hirsuta (JW), OA and T. hirsuta (JOA). The substrate added by T. hirsuta was incubated for 21 days. Chemical analysis was conducted to determine weight loss, lignin content, and cellulose from various treatments. 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