Description of Klebsiella indica sp. nov., isolated from the surface of tomato

A novel bacterial strain designated TOUT106 T was isolated from the surface of a tomato collected from the local vegetable market in Pune, India. The cells were rod shaped, Gram-stain-negative, encapsulated and non-motile. The strain TOUT106 T grows as mucoid and translucent colonies on blood agar medium and the best growth was observed at 28°C and at pH 7.0, and could tolerate up to 2% (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain TOUT106 T was placed under Salmonella clade, with close similarity to Salmonella enterica subsp. arizonae strain NCTC 8297 T (98.42%). Genome-based phylogenetic analysis revealed that the strain forms a distinct branch within the Klebsiella clade and K. michiganensis DSM25444 T and K. oxytoca NBRC105695 T were the closest neighbor. The genomic DNA G+C content of strain TOUT106 T was 53.53 mol%. The average nucleotide identity of TOUT106 T was less 86.4% with closely related members of the family Enterobacteriaceae . The major fatty acids of strain TOUT106 T were C 16:0 , C 17:0 cyclo, C 14:0 3OH/C 16:1 iso, C 14:0 , C 19:0 cyclo w8c , C 18:1 w6c/C 18:1 w7c, C 12:0 and C 16:1 w7c/C 16:1 w6c. The strain TOUT106 T showed differences in physiological, phenotypic and protein profiles by MALDI-TOF MS to its closest relatives. Based on the phenotypic including chemotaxonomic properties and phylogenetic analysis the strain TOUT106 T could be distinguished from the recognized species of the genus Klebsiella , was suggested to represent a novel species of this genus, for which the name Klebsiella indica sp. nov. is proposed. The type strain is TOUT106 T (=MCC 2901 T ).