LC-MS from FLAG-based Immunoprecipitation

LC-MS from FLAG-based Immunoprecipitation | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 6 August 2025 V1 Latest version Share on LC-MS from FLAG-based Immunoprecipitation Author : Shobith Suresh 0000-0001-6336-1418 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175449164.42580300/v1 209 views 116 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract FLAG-based immunoprecipitation is a robust and specific method for isolating epitope-tagged protein complexes from cellular lysates. Coupling this technique with LC-MS (liquid chromatography-mass spectrometry) enables the identification of protein interactors and posttranslational modifications with high sensitivity and specificity. This protocol outlines a comprehensive workflow from mammalian cell culture and FLAG immunoprecipitation to onbead tryptic digestion, peptide desalting, and submission for LC-MS analysis. It includes reagent preparation, troubleshooting tips, and notes on quality control. This method facilitates in-depth proteomic profiling of FLAG-tagged proteins and associated complexes. Supplementary Material File (lcms.pdf) Download 520.74 KB Information & Authors Information Version history V1 Version 1 06 August 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords flag immunoprecipitation lc-ms mass spectrometry peptide cleanup protein complex proteomics sample preparation tryptic digest Authors Affiliations Shobith Suresh 0000-0001-6336-1418 [email protected] View all articles by this author Metrics & Citations Metrics Article Usage 209 views 116 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Shobith Suresh. LC-MS from FLAG-based Immunoprecipitation. Authorea . 06 August 2025. DOI: https://doi.org/10.22541/au.175449164.42580300/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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