Proximity-ligation metagenomics reveals disease-specific mobilome dynamics in disrupted gut ecosystems

Proximity-ligation metagenomics reveals disease-specific mobilome dynamics in disrupted gut ecosystems | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Brief Communication Proximity-ligation metagenomics reveals disease-specific mobilome dynamics in disrupted gut ecosystems Jasmohan Bajaj, Andrew Sommer, Benjamin Auch, Alexander Khoruts This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9142184/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Distinct ecological pressures shape accumulation of antimicrobial resistance and virulence genes in the gut microbiome. Using proximity ligation shotgun metagenomics to resolve host–mobilome relationships, we analyzed microbiomes from two patient cohorts: recurrent Clostridioides difficile infection (rCDI) and cirrhosis. While rCDI reflects antibiotic-driven disruption, cirrhosis-driven microbiome changes result from altered gut physiology. We found increased chromosomal determinants of antibiotic resistance in both, but plasmid-mediated amplification was more evident in rCDI. Health sciences/Gastroenterology/Gastrointestinal diseases/Liver diseases Health sciences/Gastroenterology/Gastrointestinal diseases/Dysbiosis Health sciences/Pathogenesis/Infection Full Text Additional Declarations Yes there is potential Competing Interest. BA is an employee of Phasegenomics. No other conflicts exist. The research was approved by IRBs at the University of Minnesota and the Richmond VA medical centers. All participants gave informed consent before participating. Supplementary Files ExtendedDataMethods31626.docx Online methods Extendeddataresultstext31626.docx Human cohort summary results Extendedfigures31626labeled.pdf Extended data figures 1-8 ExtendedDataTables31626.xlsx Datasheets with cirrhosis, rCDI cohort and microbiome results Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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