Novel Fabrication of Self-healing Chitosan/Alginate porous Hydrogels with Adhesive Dopamine chemistry – based materials for improve Therapeutic Potential in Femoral Fractures

Abstract This study explores the significant impact of Polydopamine nanospheres (PDA NPs) on polymer properties, ascribed to the physical and chemical interactions between the PDA and natural polymers. It also demonstrated the PDA’s ability to modify porous structure and enhance the mechanical characteristics of Chitosan/alginate (Ch/Alg) hydrogel nanocomposites for tissue engineering scaffolds. The porous scaffold with nano surface morphology, hierarchical pore structure, appropriate porosity and superior bio-mechanical strength was successfully synthesized. The hierarchical pore structure of the synthesized PDA modified Ch/Alg-DA hydrogel based scaffolds is favorable for cell ingrowth and cell adhesion. In loading and release experiments, Ch/Alg-DA/PDA hydrogels/scaffolds were confirmed to have better adsorption ability to Ciprofloxacin (CFLX) and more steady in vitro release behavior than the other hydrogels. Osteoblast cells were cultured on the surfaces of the scaffolds for up to 3 days, and proliferation and cell viability were examined using fluorescence microscopy, Moreover, the PDA loaded Ch/Ag-DA can improve the adhesion and promote the proliferation and spontaneous growth of osteoblast cells. Even though, further research is necessary to understand all the parameters affecting biocompatibility, the as prepared Ch/Ag-DA/ PDA NPs may signified an idealistic tool for the treatment of bone deficiency and regeneration of bone tissue, among other applications.