Post-Match Changes in Biomarkers and Fatigue-Related Physical Performance Parameters in Young Amateur Soccer Players: The Effects of Fasting | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Post-Match Changes in Biomarkers and Fatigue-Related Physical Performance Parameters in Young Amateur Soccer Players: The Effects of Fasting Yakoub Fenni, Mourad Ikiouane, Khaled Kettab, Hocine Remini, Salim Zaabar This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7918040/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 27 Mar, 2026 Read the published version in BMC Sports Science, Medicine and Rehabilitation → Version 1 posted 11 You are reading this latest preprint version Abstract Background Ramadan fasting poses unique physiological challenges for athletes, particularly in hot and arid environments. Understanding its effects on post-match recovery and performance is crucial for optimizing training and competition strategies. Methods Eighteen male amateur soccer players (25.5 ± 3.2 years) from a regional club in southern Algeria were evaluated before, during, and after Ramadan. Blood biomarkers—including creatine kinase (CK), C-reactive protein (CRP), red and white blood cells (RBCs, WBCs), hemoglobin (HGB), hematocrit (HCT), and platelets (PLTs)—were measured. Physical performance was assessed using the Yo-Yo Intermittent Recovery Test Level 1 (Yo-Yo IR1), countermovement jump (CMJ), and 20 m sprint tests. Repeated-measures ANOVA and Friedman tests were used to detect differences across periods (p < 0.05). Results Significant reductions in CRP, WBCs, PLTs, HGB, and CK were observed during Ramadan compared with pre- and post-fasting periods, whereas HCT and RBCs remained stable. Yo-Yo IR1 performance declined significantly during Ramadan, while CMJ and sprint performance showed no significant changes. These findings indicate a selective reduction in aerobic capacity with maintenance of anaerobic and explosive performance. Conclusions Ramadan fasting induces measurable alterations in inflammatory, hematological, and muscle-damage markers among soccer players in hot desert conditions. Although aerobic performance is compromised, short-duration and power-based efforts remain unaffected. Tailored hydration, nutrition, and training strategies are recommended to support athlete recovery and performance during Ramadan. Ramadan fasting Biomarkers Fatigue Physical performance Soccer players Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 27 Mar, 2026 Read the published version in BMC Sports Science, Medicine and Rehabilitation → Version 1 posted Editorial decision: Revision requested 20 Jan, 2026 Reviews received at journal 03 Jan, 2026 Reviews received at journal 31 Dec, 2025 Reviewers agreed at journal 24 Dec, 2025 Reviewers agreed at journal 22 Dec, 2025 Reviewers agreed at journal 15 Dec, 2025 Reviewers invited by journal 15 Dec, 2025 Editor assigned by journal 08 Dec, 2025 Editor invited by journal 12 Nov, 2025 Submission checks completed at journal 11 Nov, 2025 First submitted to journal 11 Nov, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7918040","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":561969574,"identity":"55a6cf83-7b7e-4dc6-aeca-5867db69b5b5","order_by":0,"name":"Yakoub 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Fasting","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"
[email protected]","identity":"bmc-sports-science-medicine-and-rehabilitation","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"ssmr","sideBox":"Learn more about [BMC Sports Science, Medicine and Rehabilitation](http://bmcsportsscimedrehabil.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/ssmr/default.aspx","title":"BMC Sports Science, Medicine and Rehabilitation","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Ramadan fasting, Biomarkers, Fatigue, Physical performance, Soccer players","lastPublishedDoi":"10.21203/rs.3.rs-7918040/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7918040/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eRamadan fasting poses unique physiological challenges for athletes, particularly in hot and arid environments. Understanding its effects on post-match recovery and performance is crucial for optimizing training and competition strategies.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eEighteen male amateur soccer players (25.5\u0026thinsp;\u0026plusmn;\u0026thinsp;3.2 years) from a regional club in southern Algeria were evaluated before, during, and after Ramadan. Blood biomarkers\u0026mdash;including creatine kinase (CK), C-reactive protein (CRP), red and white blood cells (RBCs, WBCs), hemoglobin (HGB), hematocrit (HCT), and platelets (PLTs)\u0026mdash;were measured. Physical performance was assessed using the Yo-Yo Intermittent Recovery Test Level 1 (Yo-Yo IR1), countermovement jump (CMJ), and 20 m sprint tests. Repeated-measures ANOVA and Friedman tests were used to detect differences across periods (p\u0026thinsp;\u0026lt;\u0026thinsp;0.05).\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eSignificant reductions in CRP, WBCs, PLTs, HGB, and CK were observed during Ramadan compared with pre- and post-fasting periods, whereas HCT and RBCs remained stable. Yo-Yo IR1 performance declined significantly during Ramadan, while CMJ and sprint performance showed no significant changes. These findings indicate a selective reduction in aerobic capacity with maintenance of anaerobic and explosive performance.\u003c/p\u003e\u003ch2\u003eConclusions\u003c/h2\u003e \u003cp\u003eRamadan fasting induces measurable alterations in inflammatory, hematological, and muscle-damage markers among soccer players in hot desert conditions. Although aerobic performance is compromised, short-duration and power-based efforts remain unaffected. Tailored hydration, nutrition, and training strategies are recommended to support athlete recovery and performance during Ramadan.\u003c/p\u003e","manuscriptTitle":"Post-Match Changes in Biomarkers and Fatigue-Related Physical Performance Parameters in Young Amateur Soccer Players: The Effects of Fasting","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-12-18 19:12:41","doi":"10.21203/rs.3.rs-7918040/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2026-01-20T12:08:21+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-01-04T02:46:26+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-12-31T20:07:27+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"72642025261740537763303731152160745282","date":"2025-12-24T13:56:03+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"23043986454708021783822554760818570137","date":"2025-12-22T12:15:10+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"119912041445490464557148193220506626478","date":"2025-12-15T12:02:32+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-12-15T11:49:26+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-12-08T22:28:59+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2025-11-12T16:59:11+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-11-11T22:28:42+00:00","index":"","fulltext":""},{"type":"submitted","content":"BMC Sports Science, Medicine and Rehabilitation","date":"2025-11-11T22:26:03+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"
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