Antifungal Activity and the Mechanism of Ozone Against Spoilage Molds, Such as Gibberella Intermedia and Aspergillus Ochraceus Isolated From Freshly-Peeled Garlic

The antibacterial effect and mechanism of ozone (O 3 ) treatment on freshly-peeled garlic inoculated with Gibberella intermedia (G. intermedia) and Aspergillus ochraceus (A. ochraceus) in different conditions were studied in vivo . The results showed that the specific O 3 concentration, treatment time, and humidity significantly affected the garlic's indexes. The optimal treatment conditions of garlic inoculated with both molds were 6 ppm, 15 min, and 90%, respectively. After treatment with those conditions, the incidence etc of garlic were significantly reduced during storage. Differential analysis was performed for the RNA-sequencing and gene expression of the molds subjected to O 3 treatment, as well as the samples that were not. The results showed that O 3 treatment inhibited the growth of G. intermedia and A. ochraceus. Consequently, 2754 and 2378 differentially expressed genes, 1456 and 1591 up-regulated genes, and 1298 and 787 down-regulated genes were screened, respectively, for these molds, affecting the catalytic activity and various other pathways.