The Research of Site Supervision of Aids Screening Laboratory in Sichuan Province During 2011-2020: A Laboratory Capacity-based Continuity Study

Background: Expanding the scale of Human immunodeficiency virus (HIV) testing is long-term strategy for acquired immune deficiency syndrome (AIDS) prevention and control. This study analyzes the capacity of HIV screening laboratory operation to find influencing factors and guarantee quality of test. The result could also provide reference value for management of AIDS laboratories in other provinces and even abroad. Methods: Randomly checking AIDS screening laboratories in 21 cities and counties (districts) every year and also filling out evaluation form of site supervision. Analyzing the influence factors of HIV screening laboratory operation though Logistic regression. Result: From 2011 to 2020, there were 68, 75, 81, 82, 87, 80, 33, 57, 85 and 87 HIV screening laboratories conducted site supervision respectively, of which 60(88.24%), 74 (98.67%), 76 (93.83%), 81(98.78%), 60(68.97%), 51(63.75%), 23(69.70%), 41(71.93%), 59(69.41%) and 60(68.97%) had excellent results. It was found that the excellence rate of each institution was relatively stable during 2011-2014, while the overall score of each regional laboratory in 2015 fluctuated significantly than 2015 before and all of excellence rate has declined. Hospitals and centers for disease control and prevention (CDC) were with higher frequency of assessment. Multivariate logistic regression analysis found these following variables were significant influencing factors of laboratory quality, including staffing, standard operating procedure (SOP) and management system, periodic calibration of equipment, standardization of HIV testing, uniqueness of sample number, standardized use of quality control materials, standard drawing of quality control chart, biosafety facilities . Conclusion: The overall operation of HIV screening laboratories in the whole province is good and the quality management can normatively meet the requirements, which also meet the demand of expanding HIV testing. It also has lots of questions influencing laboratory quality, so each part is still strictly required to ensure laboratory work well.