An optimised method for the recovery of laboratory generated SARS-CoV-2 aerosols by plaque assay

2022 · doi:10.1101/2022.10.31.514483
preprint OA: gold CC-BY-NC-ND-4.0
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This paper describes an optimized method for recovering SARS-CoV-2 aerosols using plaque assays, which can be readily integrated into BCL3 laboratory protocols.

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Abstract

We present an optimised method for the recovery of laboratory generated SARS-CoV-2 virus by plaque assay. This method allows easy incorporation into existing standard operating procedures of biological containment level 3 (BCL3) laboratories.

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License: CC-BY-NC-ND-4.0