Food Safety for Health Promotion: Unravelling Spirulina's Antimicrobial Power in Controlling Escherichia coli and Staphylococcus aureus isolated from fresh Tilapia Fillet and AI Verification

Food Safety for Health Promotion: Unravelling Spirulina's Antimicrobial Power in Controlling Escherichia coli and Staphylococcus aureus isolated from fresh Tilapia Fillet and AI Verification | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Food Safety for Health Promotion: Unravelling Spirulina's Antimicrobial Power in Controlling Escherichia coli and Staphylococcus aureus isolated from fresh Tilapia Fillet and AI Verification Wubshet Asnake Metekia, Beyza Hatice Ulusoy, Seblewongiel Aynalem Kidanie This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4959132/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Seafood products, including fresh tilapia fish fillets, are prone to rapid quality deterioration due to microbial contamination, which is a concern for food safety and health promotion to the general public. This study focused on experimental investigation and artificial intelligence model verification of the antimicrobial activity of Spirulina platensis (SP) extracts on two common foodborne pathogens, Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). The SP extracts were obtained using the freeze-thaw method at concentrations of 0.5%, 1%, and 5% (w/v) and labeled as EA, EB, and EC, respectively. Microbial analysis was conducted from 25 fresh Nile Tilapia fish fillets. Each fresh fish was dissected into four quarters; one for the control and three were for the treatments. Bacterial count was conducted before and after the treatment with the extracts at 1 hour, 24 hours, and 48 hours with storage at 4°C. The antimicrobial activity of the extracts was modeled using Artificial Neural Network (ANN) and Adaptive Neuro Fuzzy Inference System (ANFIS) models. The results showed that the SP extracts exhibited effective antimicrobial activity against E. coli and S. aureus in fresh tilapia fish fillets. The ANN and ANFIS models accurately verified the reduction in microbial count after treatment with the extracts. Notably, EC demonstrated superior antimicrobial activity compared to EB and EA. The findings suggest that SP extracts can serve as a natural preservative technology for food products. It could be used as a strategy for food safety and health promotion to protect the public from pathogenic foodborne bacteria. Future research should focus on exploring the bioactive compounds of SP extracts in various food matrices and in super food and drug discoveries. Spirulina platensis extracts antimicrobial activity fish fillets E. coli Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4959132","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":357246293,"identity":"f669d932-c714-4477-9283-32a278636d3f","order_by":0,"name":"Wubshet Asnake Metekia","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA80lEQVRIiWNgGAWjYFACHsYDCQwMMgwMjG0MH4B8NnbCWhhAWnhAWhhngLQwE6MFTIIUg0gGQloMbuQeOPCwzYbH4Pzitsc2v7bJ8zEzMH74mINPS17CgcS2NB6DGw/bjXP7bhu2MTMwS87chluL2Y0cgwMJZw4DtRxsk87tuc0I1MLGzEtYy3+IFsue2/ZEaqk4APRLY5s0w4/biQS12J95lwDUkswjeYOx3bC34XZyGzNjM16/SLbnHnz4w8BOju/88WcPfvy5bTu/vfngh494tMCBwo0EcAIAAsYGItQDgXz/ASD5hzjFo2AUjIJRMLIAAAarWfPFsWZxAAAAAElFTkSuQmCC","orcid":"","institution":"Ethiopian Ministry of Agriculture","correspondingAuthor":true,"prefix":"","firstName":"Wubshet","middleName":"Asnake","lastName":"Metekia","suffix":""},{"id":357246294,"identity":"055c6a8d-edf5-4f93-a86f-89746c2aa826","order_by":1,"name":"Beyza Hatice Ulusoy","email":"","orcid":"","institution":"Near East University","correspondingAuthor":false,"prefix":"","firstName":"Beyza","middleName":"Hatice","lastName":"Ulusoy","suffix":""},{"id":357246295,"identity":"2428085f-e996-42bb-9504-f1f595509cf1","order_by":2,"name":"Seblewongiel Aynalem Kidanie","email":"","orcid":"","institution":"Bahir Dar University","correspondingAuthor":false,"prefix":"","firstName":"Seblewongiel","middleName":"Aynalem","lastName":"Kidanie","suffix":""}],"badges":[],"createdAt":"2024-08-22 15:29:25","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4959132/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4959132/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":66300138,"identity":"b065c6dd-bb72-4f7d-8eba-89346effe22a","added_by":"auto","created_at":"2024-10-10 05:24:57","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":399768,"visible":true,"origin":"","legend":"","description":"","filename":"MANUSCRIPTofMETEKIAetalParaphrased.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4959132/v1_covered_aab9ec0a-1d1d-4a85-b188-bba3c51a834d.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Food Safety for Health Promotion: Unravelling Spirulina's Antimicrobial Power in Controlling Escherichia coli and Staphylococcus aureus isolated from fresh Tilapia Fillet and AI Verification","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Spirulina platensis extracts, antimicrobial activity, fish fillets, E. coli","lastPublishedDoi":"10.21203/rs.3.rs-4959132/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4959132/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eSeafood products, including fresh tilapia fish fillets, are prone to rapid quality deterioration due to microbial contamination, which is a concern for food safety and health promotion to the general public. This study focused on experimental investigation and artificial intelligence model verification of the antimicrobial activity of \u003cem\u003eSpirulina platensis\u003c/em\u003e (SP) extracts on two common foodborne pathogens, \u003cem\u003eEscherichia coli\u003c/em\u003e (\u003cem\u003eE. coli\u003c/em\u003e) and \u003cem\u003eStaphylococcus aureus\u003c/em\u003e (\u003cem\u003eS. aureus\u003c/em\u003e). The SP extracts were obtained using the freeze-thaw method at concentrations of 0.5%, 1%, and 5% (w/v) and labeled as EA, EB, and EC, respectively. Microbial analysis was conducted from 25 fresh Nile Tilapia fish fillets. Each fresh fish was dissected into four quarters; one for the control and three were for the treatments. Bacterial count was conducted before and after the treatment with the extracts at 1 hour, 24 hours, and 48 hours with storage at 4\u0026deg;C. 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Future research should focus on exploring the bioactive compounds of SP extracts in various food matrices and in super food and drug discoveries.\u003c/p\u003e","manuscriptTitle":"Food Safety for Health Promotion: Unravelling Spirulina's Antimicrobial Power in Controlling Escherichia coli and Staphylococcus aureus isolated from fresh Tilapia Fillet and AI Verification","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-10-01 09:44:06","doi":"10.21203/rs.3.rs-4959132/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"cea58100-8f81-4c65-ba10-a4ac3e2ee779","owner":[],"postedDate":"October 1st, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-10-10T05:08:52+00:00","versionOfRecord":[],"versionCreatedAt":"2024-10-01 09:44:06","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4959132","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4959132","identity":"rs-4959132","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}