Alteration of the phyllosphere microbiota in lettuces by different light spectra

Alteration of the phyllosphere microbiota in lettuces by different light spectra | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Alteration of the phyllosphere microbiota in lettuces by different light spectra Shangdong Yang, Ziwei He, Yu Zhu, Lulu Qiu, Weifeng Gu, Xiao Wei, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8477401/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 8 You are reading this latest preprint version Abstract To investigate light quality how to shape phyllosphere microbial communities on crop leaves, we analyzed the microbiota of lettuces grown under five light treatments: nature light (CK), Red light (RL), Yellow light (YL), Blue light (BL) and white light (WL) using high-throughput sequencing. The results revealed that light quality significantly altered both bacterial and fungal compositions in the lettuces phyllosphere. At the genus level, in comparison with nature light (CK), RL and YL enriched bacterial genera, such as Rothia , Bradyrhizobium , and fungal genera, such as Cladosporium , Phialosimplex . Notably Rickettsia was uniquely enriched under RL, along with fungal genera Trametes and Ramichloridium . Similarly, YL specifically enriched bacterial genera Hyphomicrobium and Novosphingobium , fungal genera Myrothecium , Talaromyces and Sporobolomyces . In contrast, Exiguobacterium and Cystobasidium were enriched across CK, BL, and WL treatments, while Rhodococcus and Pseudocercospora were uniquely abundant under WL treatment. Function prediction further indicated that symbiotrophic fungi were significantly enriched under RL, whereas WL promoted the proliferation of potentially pathogenic bacteria and fungi (e.g., Pseudocercospora ). Collectively, these findings demonstrate that light quality is a key driver of phyllosphere microbial assembly in lettuce. provide new insights into light-driven microbial assembly on lettuce leaves and set the stage for functional studies on plant-microbe interactions under controlled light spectra. lettuce phyllosphere microorganisms light spectrum Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Reviewers agreed at journal 09 Mar, 2026 Reviews received at journal 03 Mar, 2026 Reviewers agreed at journal 23 Feb, 2026 Reviewers invited by journal 23 Feb, 2026 Editor invited by journal 30 Dec, 2025 Editor assigned by journal 30 Dec, 2025 Submission checks completed at journal 30 Dec, 2025 First submitted to journal 29 Dec, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8477401","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":595589199,"identity":"918602af-1993-4dc5-9eaa-bede8cbb001c","order_by":0,"name":"Shangdong 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The results revealed that light quality significantly altered both bacterial and fungal compositions in the lettuces phyllosphere. At the genus level, in comparison with nature light (CK), RL and YL enriched bacterial genera, such as \u003cem\u003eRothia\u003c/em\u003e, \u003cem\u003eBradyrhizobium\u003c/em\u003e, and fungal genera, such as \u003cem\u003eCladosporium\u003c/em\u003e, \u003cem\u003ePhialosimplex\u003c/em\u003e. Notably \u003cem\u003eRickettsia\u003c/em\u003e was uniquely enriched under RL, along with fungal genera \u003cem\u003eTrametes\u003c/em\u003e and \u003cem\u003eRamichloridium\u003c/em\u003e. Similarly, YL specifically enriched bacterial genera \u003cem\u003eHyphomicrobium\u003c/em\u003e and \u003cem\u003eNovosphingobium\u003c/em\u003e, fungal genera \u003cem\u003eMyrothecium\u003c/em\u003e, \u003cem\u003eTalaromyces\u003c/em\u003e and \u003cem\u003eSporobolomyces\u003c/em\u003e. In contrast, \u003cem\u003eExiguobacterium\u003c/em\u003e and \u003cem\u003eCystobasidium\u003c/em\u003e were enriched across CK, BL, and WL treatments, while \u003cem\u003eRhodococcus\u003c/em\u003e and \u003cem\u003ePseudocercospora\u003c/em\u003e were uniquely abundant under WL treatment. Function prediction further indicated that symbiotrophic fungi were significantly enriched under RL, whereas WL promoted the proliferation of potentially pathogenic bacteria and fungi (e.g., \u003cem\u003ePseudocercospora\u003c/em\u003e). 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