Effects of Lactobacillus plantarum P10-R10, Lactobacillus salivarius P11-R11 and Lactobacillus reuteri DSM17938 on intestinal microbiota and plasma metabolism in mice with alcohol-related liver injury

Effects of Lactobacillus plantarum P10-R10, Lactobacillus salivarius P11-R11 and Lactobacillus reuteri DSM17938 on intestinal microbiota and plasma metabolism in mice with alcohol-related liver injury | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Effects of Lactobacillus plantarum P10-R10, Lactobacillus salivarius P11-R11 and Lactobacillus reuteri DSM17938 on intestinal microbiota and plasma metabolism in mice with alcohol-related liver injury Yifan Xu, Yanxu Hao, Nan Chen, NA Lv, Xiaoxue Yuan, Baoli Zhu, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6750503/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Previous research has identified alterations in the Lactobacillus genus in alcohol-related liver disease, observed in both clinical and murine experiments. Different species of Lactobacilli exhibit distinct functions across various disease states. Understanding how different Lactobacillus species influence alcohol-related liver disease could lead to targeted therapeutic interventions that harness beneficial bacteria or mitigate harmful effects. Additionally, exploring these relationships may provide insights into personalized approaches for managing liver health in individuals with varying microbial compositions. Method An alcohol-related liver injury mice model was established based on Lieber-DeCarli model and Gao-Binge model. Three species of lactobacillus were administered for intervention, and control and model groups were set up. Samples were collected after 28 days, and the effects on liver function, intestinal microbiota and metabolites were evaluated by detecting indicators. Results Lactobacillus plantarum P10-R10 could ameliorate alcohol-induced liver injury. After the intervention of Lactobacillus plantarum P10-R10, plasma AST decreased in mice, liver oil red indicated a decrease in the degree of steatosis, and TG decreased in liver tissue. Relative abundance of beneficial bacteria such as Lactiplantibacillus , Faecalibacterium and Bifidobacterium increased while the relative abundance of harmful bacteria such as Helicobacter and Klebsiella decreased. L-carnitine and theophylline increased in plasma and were positively correlated with elevated genus. Functional implications of Lactobacillus salivaria P11-R11 and Lactobacillus reuteri DSM17938 merit further exploration. Conclusions The role of different species of Lactobacillus in ALD is different, and Lactobacillus plantarum ameliorate liver injury in mice with ALD. Full Text Additional Declarations No competing interests reported. Supplementary Files Supplenmentarymaterials.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6750503","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":484486817,"identity":"5a4e8196-6698-4299-9522-4aa1ff41fca4","order_by":0,"name":"Yifan Xu","email":"","orcid":"","institution":"Beijing Ditan Hospital","correspondingAuthor":false,"prefix":"","firstName":"Yifan","middleName":"","lastName":"Xu","suffix":""},{"id":484486818,"identity":"f6b04994-549d-4e48-aecb-235d9ab02fc6","order_by":1,"name":"Yanxu Hao","email":"","orcid":"","institution":"Beijing Ditan 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Different species of \u003cem\u003eLactobacilli\u003c/em\u003e exhibit distinct functions across various disease states. Understanding how different \u003cem\u003eLactobacillus\u003c/em\u003e species influence alcohol-related liver disease could lead to targeted therapeutic interventions that harness beneficial bacteria or mitigate harmful effects. Additionally, exploring these relationships may provide insights into personalized approaches for managing liver health in individuals with varying microbial compositions.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethod\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAn alcohol-related liver injury mice model was established based on Lieber-DeCarli model and Gao-Binge model. Three species of \u003cem\u003elactobacillus\u003c/em\u003e were administered for intervention, and control and model groups were set up. Samples were collected after 28 days, and the effects on liver function, intestinal microbiota and metabolites were evaluated by detecting indicators.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eLactobacillus plantarum\u003c/em\u003e P10-R10 could ameliorate alcohol-induced liver injury. After the intervention of \u003cem\u003eLactobacillus plantarum\u003c/em\u003e P10-R10, plasma AST decreased in mice, liver oil red indicated a decrease in the degree of steatosis, and TG decreased in liver tissue. Relative abundance of beneficial bacteria such as \u003cem\u003eLactiplantibacillus\u003c/em\u003e, \u003cem\u003eFaecalibacterium\u003c/em\u003e and \u003cem\u003eBifidobacterium\u003c/em\u003e increased while the relative abundance of harmful bacteria such as \u003cem\u003eHelicobacter\u003c/em\u003e and \u003cem\u003eKlebsiella\u003c/em\u003e decreased. 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