ALZET pump implantation in mice for chronic drug delivery to the cochlea

ALZET pump implantation in mice for chronic drug delivery to the cochlea | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article ALZET pump implantation in mice for chronic drug delivery to the cochlea Frederic Depreux, Donna Whitlon, Claus-Peter Richter This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4415276/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 17 Oct, 2025 Read the published version in Scientific Reports → Version 1 posted 9 You are reading this latest preprint version Abstract Drugs, chemical compounds, and other elements are often delivered to the ears of experimental animals to manipulate cochlear function, study how the ear works, identify drugs that prevent hearing loss, and test for ototoxicity. Delivery procedures for acute studies have been described in the literature. However, detailed information on methods that allow weeks of continuous drug delivery to mice cochleae is sparse. This paper describes a method for chronic drug delivery to the mouse cochlea. We illustrate the steps for the surgical implantation of an ALZET infusion pump and the placement of its catheter. We propose a ventral approach to the cochlea, using a surgical laser to make the cochleostomy and the placement of the pumps’ delivery ports into scala tympani or scala vestibuli. Depending on the experimental questions to be tested, the catheter can be directed toward the cochlear base, the vestibular system, or the cochlear apex. Biological sciences/Neuroscience Biological sciences/Neuroscience/Auditory system Biological sciences/Neuroscience/Auditory system/Cochlea Mouse surgery cochleostomy bullostomy chronic implantation micro-osmotic pump drug delivery Full Text Additional Declarations No competing interests reported. Supplementary Files Recoveryvideo.mov SupplementalinformationOsmoticpumpimplantation20240513final.docx Cite Share Download PDF Status: Published Journal Publication published 17 Oct, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 28 Apr, 2025 Reviews received at journal 31 Oct, 2024 Reviewers agreed at journal 20 Oct, 2024 Reviewers agreed at journal 28 Jun, 2024 Reviewers invited by journal 31 May, 2024 Editor assigned by journal 31 May, 2024 Editor invited by journal 17 May, 2024 Submission checks completed at journal 17 May, 2024 First submitted to journal 13 May, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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