Evaluation of Sida rhombifolia L roots extract on Immobilization stress induced male infertility in rats

Abstract Current study aimed to investigate protective effect of Sida rhombifolia L roots hydroalcoholic extract on immobilization stress induced male infertility in experimental rats. Male wistar rats (200-250g) were randomly divided into six groups (n=6). The male fertility enhancing activity was evaluated by sexual behavior, semen parameters, body weight, SGI, hematological parameters, vital and sexual accessory organ weight, histopathology by using standard procedure. GSH, SOD and LPO level were measured in testis tissues to evaluate antioxidant activity. The S. rhombifolia L. root hydroalcoholic extract indicated in vitro antioxidant activity on scavenging of hydroxy radicle, TAC and NO. Improvement in sperm quality i.e. count, motility, viability, Sexual behaviour, Body weight, sexual and vital organ weight, GSI, seminal fructose, Testicular cholesterol level, haematological parameters observed in treatment groups just like standard group. S. rhombifolia showed antioxidant capacity by lowering NO, MDA and elevated SOD in testis tissue. Investigations manifested that improved spermatogenesis in group treated with S. rhombifolia L in contrast with immobilization stress induced group resemblance to in silico finding study which revealed that SRR extract have adaptogenic and fertility enhancing effect.