Elucidation of the Salt Tolerance Mechanism of Rice by Profiling the Transcriptome in Sea Rice 86 Root

Abstract Background and aim In order to explore the molecular mechanism of salt tolerance of rice (Oryza sativa L）.Methods the transcriptome of 0.9% NaCl treated seawater rice roots was sequenced with Sea Rice 86 with strong salt tolerance.Results The results showed that 1055 differential genes were obtained by transcriptome sequencing, of which 691 differential genes were up-regulated and 364 differential genes were down regulated. Through go analysis of differentially expressed genes, it was found that salt stress caused differential expression of genes related to metabolic process, cell process, protein binding, catalytic activity, antioxidant activity and cell separation of Sea Rice 86, which may be Sea Rice 86 played an important role in root resistance to salt stress; The differentially expressed genes were annotated with KEGG function, The differentially expressed genes in plant hormone signal transduction, galactose metabolism, glutathione metabolism, plant pathogen interaction and glycolysis / gluconeogenesis were compared after 3 and 0 days of salt treatment (glycolysis / gluconeogenesis), phenylpropanoid biosynthesis, etc. The results of differentially expressed genes were consistent with the transcriptome data verified by qRT-PCR. There were significant differences in ion content, proline and peroxide metabolism in seawater rice roots under salt stress.The molecular mechanism of salt stress in the roots of Sea Rice 86 was deeply discussed, which provided a basis for further application in genetic engineering and molecular breeding.