Tat-NTS Suppresses The Proliferation, Migration and Invasion of Glioma Cells by Inhibiting Annexin-A1 Nuclear Translocation

Abstract Prevention of the nuclear translocation of Annexin-A1 with Tat-NTS (Trans-activator of transcription (Tat), nuclear translocation signal (NTS)) has recently been reported to alleviate neuronal injury and protect against cerebral stroke. However, the role that Tat-NTS plays in the occurrence and development of glioma still needs to be elucidated. Therefore, human glioma U87 cells were treated with various concentrations of Tat-NTS for 24 h, and cell proliferation, migration and invasion were determined with CCK-8 and Transwell assays, respectively. The results revealed that Tat-NTS significantly inhibited the nuclear translocation of Annexin-A1 in U87 cells, inhibited the proliferation, migration and invasion of U87 cells, and suppressed cell cycle regulatory protein expression and the activity and expression of MMP-2/MMP-9. Moreover, Tat-NTS reduced the level of p-p65 NF-κB in these cells. These results suggest that the Tat-NTS-induced inhibition of glioma cell proliferation, migration and invasion is closely associated with the induction of cell cycle arrest, the downregulation of MMP-2/-9 expression and activity and the suppression of the NF-κB signaling pathway. Thus, Tat-NTS may be a potential chemotherapeutic agent for the treatment of glioma.