Pleiotropic Activity of Hop Extract: Unveiling its Antimicrobial Efficacy in Oral Cavity

Pleiotropic Activity of Hop Extract: Unveiling its Antimicrobial Efficacy in Oral Cavity | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Pleiotropic Activity of Hop Extract: Unveiling its Antimicrobial Efficacy in Oral Cavity Qunli Guan, Yaozhong Wang, Jianning Mo, Shang Tang, Guotai Li, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4612258/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The extract of Hops ( Humulus lupulus L.), a herb medicine with a rich history spanning over 2000 years, has recently received extensive attention in food, health, and biomedicine. However, its reports in oral application are rare. To provide fundamental information for further clinical study in oral treatment, in this work, pleiotropic activity of hop extract including the stability, cytotoxicity, antioxidant activity, and antimicrobial activity of hop extract are investigated. The UV and thermal stability of hop extract were assessed using FT-IR analysis, while antioxidant activity was determined through experiments measuring DPPH, ABTS, and hydroxyl radical scavenging activities. Cytotoxicity was evaluated using the CCK-8 assay. Furthermore, we examined the antimicrobial activities of hop extract against four pathogenic microorganisms commonly associated with oral diseases—namely, E. faecalis, S. aureus, E. coli, and C. albicans —through inhibition zone and colony-forming unit assays. Experimental results show that hop extract has good UV and thermal stability, as well as good biological biocompatibility and antioxidant activity. Importantly, antimicrobial tests indicate that hop extract exhibits not only strong antibacterial activity against both Gram-positive and Gram-negative bacteria, but also effective antifungal ability. This study highlights the potential of hop extract as a promising antimicrobial agent for oral treatment. It serves as a crucial first step towards further applications and paves the way for the development of new treatments in oral therapies. Full Text Additional Declarations No competing interests reported. Supplementary Files Supportinginformation.doc Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4612258","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":323996543,"identity":"0b11e61f-5aed-4e2c-92df-6da6340a8389","order_by":0,"name":"Qunli Guan","email":"","orcid":"","institution":"The Affiliated Hospital of Qingdao University, Qingdao University","correspondingAuthor":false,"prefix":"","firstName":"Qunli","middleName":"","lastName":"Guan","suffix":""},{"id":323996545,"identity":"31d4626d-313c-43c7-a66e-6a0506019a28","order_by":1,"name":"Yaozhong Wang","email":"","orcid":"","institution":"Qingdao Stomatological 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However, its reports in oral application are rare. To provide fundamental information for further clinical study in oral treatment, in this work, pleiotropic activity of hop extract including the stability, cytotoxicity, antioxidant activity, and antimicrobial activity of hop extract are investigated. The UV and thermal stability of hop extract were assessed using FT-IR analysis, while antioxidant activity was determined through experiments measuring DPPH, ABTS, and hydroxyl radical scavenging activities. Cytotoxicity was evaluated using the CCK-8 assay. Furthermore, we examined the antimicrobial activities of hop extract against four pathogenic microorganisms commonly associated with oral diseases—namely, \u003cem\u003eE. faecalis, S. aureus, E. coli, \u003c/em\u003eand\u003cem\u003e C. albicans\u003c/em\u003e—through inhibition zone and colony-forming unit assays. 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